Active Immunization of Intact Mares against Gonadotropin-Releasing Hormone: Differential Effects on Secretion of Luteinizing Hormone and Follicle-Stimulating Hormone
| Autor: | L. S. Jones, F. Garza, J.J. Wiest, K. B. Ashley, Donald L. Thompson, D. R. McNeill, R. L. St. George, Dennis D. French, P. S. Mitchell |
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| Rok vydání: | 1986 |
| Předmět: |
endocrine system
medicine.medical_specialty medicine.drug_class Ovariectomy animal diseases Biological Availability Gonadotropin-releasing hormone Peptide hormone Biology Active immunization Anestrus Follicle-stimulating hormone Internal medicine medicine Animals Horses reproductive and urinary physiology Estrous cycle urogenital system Ovary Cell Biology General Medicine Luteinizing Hormone Endocrinology Reproductive Medicine Pituitary Gland Female Immunization Follicle Stimulating Hormone Gonadotropin Luteinizing hormone Pituitary Hormone-Releasing Hormones hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Biology of Reproduction. 35:347-352 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod35.2.347 |
| Popis: | Five lighthorse mares were actively immunized against gonadotropin releasing hormone (GnRH) to determine the relative importance of this hypothalamic hormone in the secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Five mares immunized against the conjugation protein served as controls. Mares were initially immunized in November and received secondary immunizations 4 wk later, and then at 6-wk intervals until ovariectomy in June. All mares immunized against GnRH exhibited an increase (p less than 0.01) in the binding of tritiated GnRH by plasma, an indication that antibodies against this hormone had been elicited. Concentrations of LH, FSH and progesterone in weekly blood samples were lower (p less than 0.05) in GnRH-immunized mares than in controls after approximately 4 mo of immunization. However, the LH concentrations were affected to a greater degree than were FSH concentrations. All five control mares exhibited normal cycles of estrus and diestrus in spring, whereas no GnRH-immunized mare exhibited cyclic displays of estrus up to ovariectomy. All mares were injected intravenously with a GnRH analog (which cross-reacted less than 0.1% with the anti-GnRH antibodies) in May, after all control mares had displayed normal estrous cycles, to characterize the response of LH and FSH in these mares; two days later, the mares were injected with GnRH. The LH response to the analog, which was assessed by net area under the curve, was lower (p less than 0.01) by approximately 99% in mares immunized against GnRH than in control mares. In contrast, the FSH response to the analog was similar for both groups.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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