Aloe vera gel extract: Safety evaluation for acute and chronic oral administration in Sprague-Dawley rats and anticancer activity in breast and lung cancer cells
| Autor: | Min Li, Weijie Xu, Jiejing Li, Chunqin Lao, Meijiao Zhang, Di Li, Xueli Tong, Junxi Du, Xiangcai Yang, Jingmian Chen |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Lung Neoplasms Cell Survival Administration Oral Antineoplastic Agents Apoptosis Breast Neoplasms Pharmacology Aloe vera Rats Sprague-Dawley Immune system Cell Line Tumor Toxicity Tests Drug Discovery medicine Animals Chronic toxicity biology Plant Extracts business.industry Cancer biology.organism_classification medicine.disease Acute toxicity Rats Plant Leaves Cancer cell Toxicity Female Plant Preparations business Drugs Chinese Herbal |
| Zdroj: | Journal of Ethnopharmacology. 280:114434 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/j.jep.2021.114434 |
| Popis: | Ethnopharmacological relevance Aloe vera (L.) Burm. f. is a typical traditional Chinese medicine (TCM) collected in the Pharmacopoeia of the People's Republic of China (version 2015). It has been traditionally used for the treatment of constipation, and its potential therapeutic activities have been widely evaluated, including anti-tumor, anti-inflammatory and immune regulatory effects. The wide application of Aloe vera in food and therapy has raised safety issues and there are multiple safety assessments with a diverse toxicity and adverse effects from clinics and animals. Aim of the study This study aimed to investigate the safety of Aloe vera barbadensis extract C (AVBEC) in rats and analyze its anticancer activity in cell lines. Materials and methods We administrated AVBEC orally in an acute toxicity study and a 6-month chronic toxicity study to observe and confirm its safety in Sprague-Dawley (SD) rats. Additionally, we explored the cytotoxicity of AVBEC in cancer cells and non-cancer cells. We further investigated the anti-tumor activity of AVBEC, and in the meantime, probed the function of component from AVBEC. Results No deaths or substance-relative toxicity were observed in the acute toxicity study or the 6-month chronic toxicity study with doses of 44.8 g·kg-1 and 4.48 g·kg-1, respectively. In the chronic toxicity study, AVBEC did not cause organ toxicity, including crucial organ structure and chemical function, and peripheral and central immune system damage. Additionally, we found that AVBEC could induce cancer cell apoptosis with a relatively higher apoptotic ratio than in non-cancer cells by decreasing adenosine triphosphate (ATP) concentration and enhancing reactive oxygen species (ROS) production. We also identified components in AVBEC using high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) and probed the function of malic acid. This demonstrated that under the same circumstances, malic acid induced cell necrosis in cancer cells and non-cancer cells, while AVBEC did not. Conclusions These results reveal a novel mechanism of aloe gel extract in regulating cancer cell apoptosis via modulating the mitochondrial metabolism and imply a possible application of AVBEC for the treatment of malignant cancer with the safety evaluation from rats and anticancer investigation from cancer cells and non-cancer cells. |
| Databáze: | OpenAIRE |
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