Association of functional polymorphisms in the MxA gene with susceptibility to enterovirus 71 infection
| Autor: | Xiaodan Chen, Xiao-Ai Zhang, Xiujun Li, Li-Juan Liu, Wei Liu, Wu-Chun Cao, Xianjun Wang, Pan-He Zhang, Cui He, Tingyu Li, Hong Yang, Shujun Ding, Lu Zhuang, Renli Zhang, Hong-Mei Xu, Hao Li |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Myxovirus Resistance Proteins China Genotype Population Allele Carrier Real-Time Polymerase Chain Reaction Severity of Illness Index Feces Risk Factors Polymorphism (computer science) Enterovirus Infections Genetics Enterovirus 71 Humans EV71 Infection Genetic Predisposition to Disease RNA Messenger Allele education Genetics (clinical) Original Investigation education.field_of_study Polymorphism Genetic EV71 Outbreak biology Southern Population Haplotype Infant Sequence Analysis DNA Northern Population biology.organism_classification Virology Enterovirus A Human Real-time polymerase chain reaction Gene Expression Regulation Haplotypes Child Preschool Immunology Female Restriction fragment length polymorphism Sentinel Surveillance |
| Zdroj: | Human Genetics |
| ISSN: | 1432-1203 0340-6717 |
| DOI: | 10.1007/s00439-013-1367-3 |
| Popis: | Myxovirus resistance A (MxA) is an antiviral protein induced by type I interferons α and β (IFN-α and IFN-β) that can inhibit virus replication. We examined whether the MxA polymorphisms were related to the risk and severity of enterovirus 71 (EV71) infection in Chinese populations. The MxA C-123A and G-88T polymorphisms were genotyped in two independent case–control populations in China by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) analysis. Multivariate logistic regression analysis was used to calculate adjusted odds ratios (ORs) and 95 % confidence intervals (95 % CIs). MxA messenger RNA was quantified by real-time quantitative PCR in peripheral blood mononuclear cells (PBMCs) from 45 healthy children and 19 patients with EV71 infection. Significantly decreased susceptibility to EV71 infection was observed for the -123A allele and -88T allele carriers, with ORs (95 % CIs) estimated as 0.56 (0.39–0.81) and 0.64 (0.47–0.88), respectively, in the northern population. This association was confirmed in the southern population, with ORs (95 % CIs) estimated as 0.58 (0.38–0.89) and 0.67(0.47–0.95), respectively. The A- 123T- 88 haplotype was also significantly associated with lower risk of EV71 infection in both the northern (OR = 0.62; 95 % CI = 0.44–0.85) and the southern population (OR = 0.63; 95 % CI = 0.43–0.92). Furthermore, we observed higher MxA messenger RNA levels in IFNβ1a-stimulated PBMCs from the -123A or -88T allele carriers compared with that from nocarriers. Our findings suggest that polymorphisms in the MxA promoter may play a role in mediating the susceptibility to EV71 infection in Chinese population. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink