Chemical Constituents from Cimicifuga dahurica and Their Anti-Proliferative Effects on MCF-7 Breast Cancer Cells
| Autor: | Ghulam Jilany Khan, Chu Thi Thanh Huyen, Bui Thi Thuy Luyen, Ha Van Oanh, Hui-Jun Li, Ta Manh Hung, Ping Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
lignan
Cimicifuga Cimicifuga dahurica phenolic amide triterpenoid glycoside anti-proliferation Pharmaceutical Science Ranunculaceae Breast Neoplasms Pharmacology 01 natural sciences Article Lignans Analytical Chemistry lcsh:QD241-441 chemistry.chemical_compound Cyclin D1 lcsh:Organic chemistry Western blot Phenols Drug Discovery medicine Humans Physical and Theoretical Chemistry Cell Proliferation Lignan chemistry.chemical_classification medicine.diagnostic_test biology 010405 organic chemistry Plant Extracts Organic Chemistry Glycoside biology.organism_classification In vitro 0104 chemical sciences 010404 medicinal & biomolecular chemistry MCF-7 chemistry Phytochemical Chemistry (miscellaneous) MCF-7 Cells Molecular Medicine Female |
| Zdroj: | Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry Molecules; Volume 23; Issue 5; Pages: 1083 Molecules, Vol 23, Iss 5, p 1083 (2018) |
| ISSN: | 1420-3049 |
| Popis: | This study was designed to search for novel anti-cancer compounds from natural plants. The 70% ethanolic extract from the rizhomes of Cimicifuga dahurica (Turcz.) Maxim. (Ranunculaceae) was found to possess significant in vitro anti-proliferative effects on MCF-7 breast cancer cells. A phytochemical investigation using assay-guided fractionation of the ethanolic extract of C. dahurica resulted in the isolation of one new phenolic amide glycoside 3, two new lignan glycosides 4 and 7, one new 9,19-cycloartane triterpenoid glycoside 6, and thirteen known constituents 1, 2, 5, and 8–17. The structures of 3, 4, 6, and 7 were established using contemporary NMR methods and from their HRESIMS data. The anti-proliferative effects of isolated compounds were evaluated using the BrdU-proliferation kit. Five among the 17 isolated compounds showed significant anti-proliferative effects (p ≤ 0.05), wherein compound 7 showed the most significant anti-proliferative and cell cycle arresting effect (p ≤ 0.05) which followed a dose dependent manner. Western blot protein expression analysis showed a down expression of c-Myc and cyclin D1 which further elucidated the anti-proliferation mechanism of compound 7 while apoptotic effects were found in association with Bcl-2 family protein expression variations. Conclusively this study reports the isolation and identification of 17 compounds from C. dahurica, including four novel molecules, in addition to the fact that compound 7 possesses significant anti-proliferative and apoptotic effects in vitro that may require further exploration. |
| Databáze: | OpenAIRE |
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