How will farmed populations of freshwater fish deal with the extreme climate scenario in 2100? Transcriptional responses of Colossoma macropomum from two Brazilian climate regions
| Autor: | Carlos Henrique dos Anjos dos Santos, Vera Maria Fonseca de Almeida-Val, Adalberto Luis Val, Luciana Mara Fé-Gonçalves, José Deney Alves Araújo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Fish Proteins Thermotolerance Physiology 030310 physiology Tambaqui Fish farming Population Fisheries Climate change 010603 evolutionary biology 01 natural sciences Biochemistry Global Warming Transcriptome 03 medical and health sciences Genetic variation Animals Protein Interaction Maps education Ecosystem 0303 health sciences education.field_of_study biology Ecology biology.organism_classification Protein ubiquitination Freshwater fish Characiformes General Agricultural and Biological Sciences Brazil Developmental Biology |
| Zdroj: | Journal of thermal biology. 89 |
| ISSN: | 0306-4565 |
| Popis: | Tambaqui (Colossoma macropomum Cuvier, 1818) is an endemic fish of the Amazon and Orinoco basins, and it is the most economically important native species in Brazil being raised in five climatically distinct regions. In the face of current global warming, environmental variations in farm ponds represent additional challenges that may drive new adaptive regional genetic variations among broodstocks of tambaqui. In an experimental context based on the high-emission scenario of the 5th Intergovernmental Panel on Climate Change (IPCC) report, we used two farmed tambaqui populations to test this hypothesis. RNA-seq transcriptome analysis was performed in the liver of juvenile tambaqui from northern (Balbina Experimental Station, Balbina, AM) and southeastern (Brumado Fish Farming, Mogi Mirim, SP) Brazilian regions kept for 30 days in artificial environmental rooms mimicking the current and extreme climate scenarios. Three Illumina MiSeq runs produced close to 120 million 500 bp paired-end reads; 191,139 contigs were assembled with N50 = 1595. 355 genes were differentially expressed for both populations in response to the extreme scenario. After enrichment analysis, each population presented a core set of genes to cope with climate change. Northern fish induced genes related to the cellular response to stress, activation of MAPK activity, response to unfolded protein, protein metabolism and cellular response to DNA damage stimuli. Genes biologically involved in regulating cell proliferation, protein stabilisation and protein ubiquitination for degradation through the ubiquitin-proteasome system were downregulated. Genes associated with biological processes, including the cellular response to stress, MAPK cascade activation, homeostatic processes and positive regulation of immune responses were upregulated in southeastern fish. The downregulated genes were related to cytoskeleton organisation, energy metabolism, and the regulation of transcription and biological rhythms. Our findings reveal the signatures of promising candidate genes involved in the regional plasticity of each population of tambaqui in dealing with upcoming climate changes. |
| Databáze: | OpenAIRE |
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