Cellular response to mineral trioxide aggregate
| Autor: | Mahmoud Torabinejad, Fraser McDonald, Eng Tiong Koh, Thomas R. Pitt Ford |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Mineral trioxide aggregate
Pathology medicine.medical_specialty Materials science medicine.medical_treatment Enzyme-Linked Immunosorbent Assay law.invention Root Canal Filling Materials law Bone cell Tumor Cells Cultured medicine Humans Methylmethacrylates Macrophage Cementogenesis Zinc Oxide-Eugenol Cement Aluminum Compounds General Dentistry Dental Cementum Osteoblasts Interleukin-6 Macrophage Colony-Stimulating Factor Silicates Petri dish Interleukin Substrate (chemistry) Oxides Biological activity Calcium Compounds Molecular biology Drug Combinations Cytokine Microscopy Electron Scanning Interleukin-1 |
| Zdroj: | Journal of Endodontics. 24:543-547 |
| ISSN: | 0099-2399 |
| Popis: | This investigation studied the cytomorphology of osteoblasts in the presence of Mineral Trioxide Aggregate (MTA) and examined cytokine production. MTA and Intermediate Restorative Material (IRM) were prepared and placed in separate Petri dishes. Osteoblasts (cell-line MG-63), grown to confluence in Hams F12/Dulbecco's modified Eagle's medium, were seeded into the dishes, which were incubated for 1 to 7 days. The specimens were viewed by scanning electron microscopy. For cytokine evaluation, cells were grown either alone or in other dishes containing the test materials for 1 to 144 h. Media were removed for ELISA analysis of interleukin (IL)-1 alpha, IL-1 beta, IL-6, and macrophage colony-stimulating factor. Scanning electron microscopy revealed healthy cells in contact with MTA at 1 and 3 days; in contrast, cells in the presence of IRM appeared rounded. The ELISA assays revealed raised levels of all ILs at all periods when cells were grown in the presence of MTA; in contrast, cells grown alone or with IRM produced undetectable amounts. The macrophage colony-stimulating factor was produced by cells irrespective of the group. It seems that MTA offers a biologically active substrate for bone cells and stimulates IL production. |
| Databáze: | OpenAIRE |
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