Production of inbred progenies of diploid potatoes using an S-locus inhibitor (Sli) gene, and their characterization
| Autor: | K Hosaka, R K Birhman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
Male
Solanum chacoense Genotype Genes Plant Inbred strain Genetics Animals Inbreeding Allele Molecular Biology Gene Crosses Genetic Phylogeny Solanaceae biology fungi food and beverages Selfing General Medicine biology.organism_classification Diploidy Seeds Pollen Restriction fragment length polymorphism Ploidy Polymorphism Restriction Fragment Length Biotechnology |
| Zdroj: | Genome. 43(3):495-502 |
| ISSN: | 0831-2796 |
| Popis: | To develop inbred lines from self-incompatible, cultivated diploid potatoes, an S-locus inhibitor (Sli) gene derived from a self-compatible variant of a wild potato species, Solanum chacoense, was incorporated into various cultivated diploid potatoes. The progeny was selfed twice by the action of the Sli gene to obtain 74 S2inbred clones belonging to 8 families. More than 40% of them were either non-flowering or pollen sterile. Among the pollen fertile clones, self-compatible clones occurred with a much lower frequency (20.9%) than expected (83.3%). The result demonstrated that self-compatibility was introduced and expressed in the gene pool of cultivated diploid potatoes by an action of the Sli gene, although serious inbreeding depression associated with selfing occurred. The genotypes of S2inbreds were surveyed using 46 S. chacoense - specific RFLP (restriction fragment length polymorphism) markers covering the whole potato genome. More than half of the markers (67.4%) showed distorted segregation. Particularly, all markers on chromosome 12 were overrepresented in the S2inbreds. This confirms our earlier finding that the Sli gene locates on chromosome 12 and the alleles linked with this gene are preferentially transmitted because of its essential requirement for selfing.Key words: RFLP markers, inbreds, diploid potato, distorted segregation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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