Gene expression changes in human cells after exposure to mobile phone microwaves

Autor: Reetta Nylund, Isabelle Lagroye, Richard Gminski, M. Angeles Trillo, Claudio Franceschi, Dariusz Leszczynski, Niels Kuster, Jukka Reivinen, Daniel Remondini, Kathrin Schlatterer, Christian Maercker, Rudolf Fitzner, Florence Poulletier de Gannes, Miriam Capri, Ferdinando Bersani, Bernard Veyret, Rudolf Tauber, E. Haro, Jürgen Schuderer
Přispěvatelé: Department of Physics, Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), Radiation and Nuclear Safety Authority Department of Research and Environmental Surveillance (STUK), STUK, Laboratoire de physique des interactions ondes matières (LPIOM), Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Bioélectromagnétisme, École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB), Hospital Universitario Ramón y Cajal [Madrid], Universidad de Alcalá - University of Alcalá (UAH), Centro Interdipartimentale Galvani (CIG), Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], IT'IS Foundation (IT'IS), IT'IS Foundation, German resource center for genome Research (RZPD), RZPD German resource center for genome Research, Mannheim University Of Applied Sciences, Hochschule Mannheim - University of Applied Sciences, Remondini D., Nylund R., Reivinen J., Poulletier de Gannes F., Veyret B., Lagroye I., Haro E., Trillo M.A., Capri M., Franceschi C., Schlatterer K., Gminski R., Fitzner R., Tauber R., Schuderer J., Kuster N., Leszczynski D., Bersani F., Maercker C., Taxile, Murielle
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Zdroj: Proteomics
Proteomics, Wiley-VCH Verlag, 2006, 6 (17), pp.4745-4754
ISSN: 1615-9853
1615-9861
Popis: International audience; Possible biological effects of mobile phone microwaves were investigated in vitro. In this study, which was part of the 5FP EU project REFLEX (Risk Evaluation of Potential Environmental Hazards From Low-Energy Electromagnetic Field Exposure Using Sensitive in vitroMethods), six human cell types, immortalized cell lines and primary cells, were exposed to 900 and 1800 MHz. RNA was isolated from exposed and sham-exposed cells and labeled for transcriptome analysis on whole-genome cDNA arrays. The results were evaluated statistically using bioinformatics techniques and examined for biological relevance with the help of different databases. NB69 neuroblastoma cells, T lymphocytes, and CHME5 microglial cells did not show significant changes in gene expression. In EA.hy926 endothelial cells, U937 lymphoblastoma cells, and HL-60 leukemia cells we found between 12 and 34 up- or down-regulated genes. Analysis of the affected gene families does not point towards a stress response. However, following microwave exposure, some but not all human cells might react with an increase in expression of genes encoding ribosomal proteins and therefore up-regulating the cellular metabolism.
Databáze: OpenAIRE
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