Generic solid phase extraction–liquid chromatography–tandem mass spectrometry method for fast determination of drugs in biological fluids
| Autor: | Rob J. Vreeken, Bert Ooms, William D van Dongen, Dick van de Lagemaat, Anniek Schellen |
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| Rok vydání: | 2003 |
| Předmět: |
Sulfamerazine
Chromatography Chemistry Clinical Biochemistry Reproducibility of Results Cell Biology General Medicine Tandem mass spectrometry Mass spectrometry Sensitivity and Specificity Biochemistry High-performance liquid chromatography Mass Spectrometry Orders of magnitude (mass) Analytical Chemistry Pharmaceutical Preparations Liquid chromatography–mass spectrometry medicine Humans Spectrophotometry Ultraviolet Solid phase extraction Quantitative analysis (chemistry) Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B. 788:251-259 |
| ISSN: | 1570-0232 |
| Popis: | A generic method was developed for the fast determination of a wide range of drugs in serum or plasma. The methodology comprises generic solid-phase extraction, on-line coupled to gradient HPLC with tandem mass spectrometric detection (SPE-LC-MS/MS). The individual components of the SPE-LC-MS/MS system were optimized in an integrated approach to maximize the application range and minimize the method development time. The optimized generic SPE-LC-MS/MS protocol was evaluated for 11 drugs with different physicochemical properties. Good quantification for 10 out of 11 of the pharmaceuticals in serum or plasma could be readily achieved. The quantitative assays gave recoveries better than 95%, lower quantification limits of 0.2-2.0 ng/ml, acceptable precision and accuracy and good linearity over 2-4 orders of magnitude. Carry-over was determined to be in the range of 0.02-0.10%, without optimization. © 2003 Elsevier Science B.V. All rights reserved. Chemicals/CAS: caffeine, 30388-07-9, 58-08-2; carbamazepine, 298-46-4, 8047-84-5; paclitaxel, 33069-62-4; paracetamol, 103-90-2; procainamide, 51-06-9, 614-39-1; propranolol, 13013-17-7, 318-98-9, 3506-09-0, 4199-09-1, 525-66-6; ranitidine, 66357-35-5, 66357-59-3; sulfadiazine, 547-32-0, 68-35-9; sulfamerazine, 127-58-2, 127-79-7; theobromine, 83-67-0; theophylline, 58-55-9, 5967-84-0, 8055-07-0, 8061-56-1, 99007-19-9; Pharmaceutical Preparations |
| Databáze: | OpenAIRE |
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