Identification and functional analysis of brassicicene C biosynthetic gene cluster in Alternaria brassicicola

Autor:	Naoto Tajima, Nobuo Kato, Tomonobu Toyomasu, Yusuke Higuchi, Tohru Dairi, Takeshi Sassa, Atsushi Minami
Rok vydání:	2008
Předmět:	Chromatography Gas Chemistry Pharmaceutical Clinical Biochemistry Pharmaceutical Science Biology Biochemistry Genetic analysis Genome chemistry.chemical_compound Biosynthesis Drug Discovery Gene cluster Molecular Biology Gene Chromatography High Pressure Liquid Alternaria brassicicola Cell-Free System Models Genetic Plant Extracts Organic Chemistry Alternaria Methyltransferases biology.organism_classification Culture Media Metabolic pathway chemistry Models Chemical Genes Bacterial Drug Design Multigene Family Molecular Medicine Electrophoresis Polyacrylamide Gel Diterpenes Function (biology)
Zdroj:	Bioorganicmedicinal chemistry letters. 19(3)
ISSN:	1464-3405
Popis:	The biosynthetic gene cluster of brassicicene C was identified in Alternaria brassicicola strain ATCC 96836 from genome database search. In vivo and in vitro study clearly revealed the function of Orf8 and Orf6 as a fusicoccadiene synthase and methyltransferase, respectively. The understanding toward the biosynthetic pathway promises construction of this type of diterpene compounds with genetic engineering.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5756805a7f12056de79048d29b083d93 https://pubmed.ncbi.nlm.nih.gov/19097780 Zobrazit plný text záznamu Full Text from ScienceDirect