Calcium Oxalate-Crystal Membrane Interactions: Dependence on Membrane Lipid Composition

Autor:	Jack G. Kleinman, John H. Wiessner, Neil S. Mandel, Michael W. Bigelow
Rok vydání:	1996
Předmět:	Kidney Supersaturation business.industry Urology Calcium oxalate Phospholipid Cell membrane chemistry.chemical_compound Red blood cell Membrane medicine.anatomical_structure chemistry Biochemistry Membrane fluidity Medicine business
Zdroj:	Journal of Urology. 155:1094-1098
ISSN:	1527-3792 0022-5347
DOI:	10.1016/s0022-5347(01)66398-5
Popis:	Purpose: Urolithiasis is clearly a multifaceted process, progressing from urine supersaturation to the formation of mature renal calculi. Retention of microcrystals by the urothelium is a critical event in stone maturation. Membrane phospholipids appear to be involved in the attachment of stone crystals to kidney epithelium.Materials and Methods: The current study quantitates crystal-membrane interactions following selective changes in the red blood cell (RBC) membrane phospholipid composition by using a crystal-induced membranolytic assay.Results: Membrane enrichment with anionic phospholipids was found to greatly increase crystal-membrane interactions. Crystal-membrane interaction was associated with an increase in the negative charge on the RBC membrane surface.Conclusions: Specific membrane compositions seem to facilitate the formation of a crystal attachment region on the RBC surface that is necessary for effective crystal attachment to the cell membrane.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57559d185c07ed209595d2f5d4df8acc https://doi.org/10.1016/s0022-5347(01)66398-5 Zobrazit plný text záznamu