Mutagenesis of the Virion-Sense Open Reading Frames of Tomato Leaf Curl Geminivirus
| Autor: | M. Ali Rezaian, Justin E. Rigden, Ian B. Dry, Philip M. Mullineaux |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
viruses
Restriction Mapping Mutant DNA Single-Stranded Mutagenesis (molecular biology technique) Biology Virus Replication Virus Plant Viruses Frameshift mutation Open Reading Frames chemistry.chemical_compound Virology ORFS Frameshift Mutation Gene Sequence Deletion Genetics fungi Virion Defective Viruses food and beverages Blotting Southern Open reading frame chemistry Mutagenesis Chromosome Inversion DNA Viral DNA |
| Zdroj: | Virology. 193:1001-1005 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.1993.1215 |
| Popis: | A series of frame shift, deletion, and inversion mutants in the virion-sense open reading frames (ORFs) of the monopartite geminivirus tomato leaf curl virus have been constructed and their ability to replicate, produce single-stranded DNA, spread, and cause symptoms in tomato plants has been investigated. Disruptions in the Vl ORF lead to symptomless, systemic infections with a reduced titer of all viral DNA forms while interruptions in the V2 (coat protein) ORF disrupted spread of the virus. Mutagenesis of the virion-sense ORFs did not affect the replication of viral double-stranded DNA, although both V1 and V2 products appear to play a role in the accumulation of viral single-stranded DNA. |
| Databáze: | OpenAIRE |
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