Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation

Autor: Lucía Natalia Moro, Santiago Gabriel Miriuka, Cyntia Aban, Antonella Lombardi, María Celeste Biani, Alejandra Guberman, Marcela Nilda García, Alejandro La Greca, Gustavo Sevlever, Claudia Solari, Verónica Alejandra Furmento, Carlos Luzzani
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Pluripotent Stem Cells
Proteomics
Stromal cell
Clinical Biochemistry
PROTEOMIC
lcsh:Medicine
Biology
medicine.disease_cause
Biochemistry
Regenerative medicine
Cell Line
Extracellular matrix
purl.org/becyt/ford/1 [https]
lcsh:Biochemistry
Ciencias Biológicas
03 medical and health sciences
Extracellular Vesicles
Tandem Mass Spectrometry
microRNA
Protein targeting
medicine
Humans
lcsh:QD415-436
Wharton Jelly
Induced pluripotent stem cell
purl.org/becyt/ford/1.6 [https]
Molecular Biology
Ciencias Exactas
Principal Component Analysis
Mesenchymal stem cell
lcsh:R
EXTRACELLULAR VESICLES
Proteomic
Cell Differentiation
Mesenchymal Stem Cells
MESENCHYMAL STEM CELLS
Bioquímica y Biología Molecular
Cell biology
030104 developmental biology
DIFFERENTIATION
Differentiation
Proteome
Molecular Medicine
Stromal Cells
CIENCIAS NATURALES Y EXACTAS
Zdroj: Experimental and Molecular Medicine, Vol 50, Iss 9, Pp 1-12 (2018)
SEDICI (UNLP)
Universidad Nacional de La Plata
instacron:UNLP
CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Popis: Mesenchymal stem/stromal cells (MSCs) obtained from pluripotent stem cells (PSCs) constitute an interesting alternative to classical MSCs in regenerative medicine. Among their many mechanisms of action, MSC extracellular vesicles (EVs) are a potential suitable substitute for MSCs in future cell-free-based therapeutic approaches. Unlike cells, EVs do not elicit acute immune rejection, and they can be produced in large quantities and stored until ready to use. Although the therapeutic potential of MSC EVs has already been proven, a thorough characterization of MSC EVs is lacking. In this work, we used a label-free liquid chromatography tandem mass spectrometry proteomic approach to identify the most abundant proteins in EVs that are secreted from MSCs derived from PSCs (PD-MSCs) and from their parental induced PSCs (iPSCs). Next, we compared both datasets and found that while iPSC EVs enclose proteins that modulate RNA and microRNA stability and protein sorting, PD-MSC EVs are rich in proteins that organize extracellular matrix, regulate locomotion, and influence cell–substrate adhesion. Moreover, compared to their respective cells, iPSCs and iPSC EVs share a greater proportion of proteins, while the PD-MSC proteome appears to be more specific. Correlation and principal component analysis consistently aggregate iPSCs and iPSC EVs but segregate PD-MSC and their EVs. Altogether, these findings suggest that during differentiation, compared with their parental iPSC EVs, PD-MSC EVs acquire a more specific set of proteins; arguably, this difference might confer their therapeutic properties.
Facultad de Ciencias Médicas
Consejo Nacional de Investigaciones Científicas y Técnicas
Databáze: OpenAIRE
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