DETERMINAÇÃO DE MERCÚRIO EM FÍGADO DE TETRÁPODES MARINHOS POR ESPECTROMETRIA DE FLUORESCÊNCIA ATÔMICA ACOPLADA A GERAÇÃO DE VAPOR FRIO (CV-AFS) E ESPECTROMETRIA DE MASSA COM FONTE DE PLASMA INDUTIVAMENTE ACOPLADO (ICP-MS): UMA COMPARAÇÃO SISTEMÁTICA ENTRE AS DUAS TÉCNICAS
| Autor: | Lucia Maria de Souza de Oliveira, Ézio Sargentini Junior, Siomara D. da Rocha, Karen Silva Luko, Amauri Antonio Menegário, Lucas Pellegrini Elias, Raiza F. da Silva, Jorge Henrique Pedrobom, Everton Tiago Sulato |
|---|---|
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Química Nova v.44 n.1 2021 Química Nova Sociedade Brasileira de Química (SBQ) instacron:SBQ Química Nova, Vol 44, Iss 1, Pp 64-69 (2021) |
| ISSN: | 1678-7064 0100-4042 |
| DOI: | 10.21577/0100-4042.20170675 |
| Popis: | DETERMINATION OF MERCURY IN LIVER OF MARINE TETRAPODS BY COLD VAPOR ATOMIC FLUORESCENCE SPECTROMETRY AND INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY IN BIOLOGICAL SAMPLES: A SYSTEMATIC COMPARISON BETWEEN THE TWO TECHNIQUES. This study compares the techniques cold vapour atomic fluorescence spectrometry (CV-AFS) and inductively coupled plasma mass spectrometry (ICP-MS) for the determination of Hg in biological samples (hepatic tissue from marine tetrapods). Acid digestions were performed for the samples and aliquots of them were analysed by ICP-MS and CV-AFS. To minimize the Hg memory effect in its determination by ICP-MS, gold (200 μg L-1 in 5% v v-1 HCl) was added to the sample digests. The detection limits were 0.02 mg kg-1 for ICP-MS and 0.01 mg kg-1 for CV-AFS. Accuracy was assessed by the calculation of the recovery obtained from TORT-3 (lobster hepatopancreas) certified reference material analysis. Accurate results were obtained for both CV-AFS and ICP-MS. The comparison of the results obtained for hepatic tissue with and without lyophilization showed a significant difference among them (at 95% of confidence level), evidencing losses of Hg in the lyophilization process. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|