The use of polymerized antisera for the determination of human placental alkaline phosphatase in pregnancy sera
| Autor: | A.Fernandez De Castro, F.M. Yeager, M. Usatei-Gomez |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Electrophoresis
Immunodiffusion Hot Temperature Time Factors Polymers Placenta Clinical Biochemistry Biology Biochemistry Isozyme Pregnancy medicine Methods Animals Humans Centrifugation Cellulose Incubation Immunoelectrophoresis Antiserum Goats Immune Sera Biochemistry (medical) General Medicine medicine.disease Alkaline Phosphatase Molecular biology Precipitin Tests Pregnancy serum Antibodies Anti-Idiotypic Isoenzymes Placental alkaline phosphatase Solubility Evaluation Studies as Topic Alkaline phosphatase Female Rabbits |
| Zdroj: | Clinica chimica acta; international journal of clinical chemistry. 46(4) |
| ISSN: | 0009-8981 |
| Popis: | 1. I. Partially inhibitory antibodies against highly purified human placental alkaline phosphatase were prepared in rabbits. The specificity and sensitivity of immunochemical reactions were employed to develop a specific, reliable assay for the placental isoenzyme in pregnancy sera. 2. II. Monospecific antiserum to the placental isoenzyme was polymerized using ethylchloroformate. Incubation of pregnancy serum with the polymerized antiserum for 30 min resulted in over 90% binding of the placental isoenzyme to the surface of the antiserum, which can then be removed by centrifugation or filtration. |
| Databáze: | OpenAIRE |
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