Multiple Regions of Kaposi’s Sarcoma-Associated Herpesvirus ORF59 RNA are Required for Its Expression Mediated by Viral ORF57 and Cellular RBM15
| Autor: | Vladimir Majerciak, Maria J. Massimelli, David J. Liewehr, Zhi-Ming Zheng, Seth M. Steinberg, Jeong-Gu Kang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
lcsh:QR1-502
RNA Viral/chemistry RNA Transport lcsh:Microbiology ORF57 ORF59 Transcription (biology) Viral Regulatory and Accessory Proteins Viral Cells Cultured Herpesvirus 8 Human/genetics Cultured Human/genetics Communication Herpesviridae Infections/metabolism RNA-Binding Proteins Viral Proteins/chemistry Herpesviridae Infections Non-coding RNA RNA silencing Infectious Diseases RNA accumulation RNA editing Herpesvirus 8 Human RNA Viral Human Gene Expression Regulation Viral RNA export Cells Molecular Sequence Data RNA-dependent RNA polymerase RBM15 KSHV Biology Response Elements Microbiology Cell Line Viral Regulatory and Accessory Proteins/chemistry Viral Proteins Genetic Virology Genetics RNA polymerase I Viral/chemistry Humans Herpesvirus 8 Base Sequence Intron RNA Epistasis Genetic Molecular biology Gene Expression Regulation Protein Biosynthesis Epistasis Nucleic Acid Conformation RNA-Binding Proteins/metabolism |
| Zdroj: | Viruses, Vol 7, Iss 2, Pp 496-510 (2015) Viruses Massimelli, MJ; Majerciak, V; Kang, JG; Liewehr, DJ; Steinberg, SM; & Zheng, ZM. (2015). Multiple regions of Kaposi’s Sarcoma-associated herpesvirus ORF59 RNA are required for its expression mediated by viral ORF57 and cellular RBM15. Viruses, 7(2), 496-510. doi: 10.3390/v7020496. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/92x0j507 Viruses, vol 7, iss 2 Viruses, 7(2), 496-510. Multidisciplinary Digital Publishing Institute (MDPI) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v7020496. |
| Popis: | © 2015 by the authors; licensee MDPI, Basel, Switzerland. KSHV ORF57 (MTA) promotes RNA stability of ORF59, a viral DNA polymerase processivity factor. Here, we show that the integrity of both ORF59 RNA ends is necessary for ORF57-mediated ORF59 expression and deletion of both 5’ and 3’ regions, or one end region with a central region, of ORF59 RNA prevents ORF57-mediated translation of ORF59. The ORF59 sequence between nt 96633 and 96559 resembles other known MTA-responsive elements (MREs). ORF57 specifically binds to a stem-loop region from nt 96596–96572 of the MRE, which also binds cellular RBM15. Internal deletion of the MRE from ORF59 led to poor export, but accumulation of nuclear ORF59 RNA in the presence of ORF57 or RBM15. Despite of being translatable in the presence of ORF57, this deletion mutant exhibits translational defect in the presence of RBM15. Together, our results provide novel insight into the roles of ORF57 and RBM15 in ORF59 RNA accumulation and protein translation. |
| Databáze: | OpenAIRE |
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