Wild-type cutoff for Apramycin against Escherichia coli

Autor:	Jiarui Li, Fulei Li, Ruimeng Liu, Hongxiao Yu, Tianshi Xiao, Yuqi Yang, Ishfaq Muhammad, Ping Cheng, Xiuying Zhang
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Swine 040301 veterinary sciences Aac(3)-IV Veterinary clinics Resistance Prevalence Microbial Sensitivity Tests Biology Apramycin medicine.disease_cause Microbiology 0403 veterinary science 03 medical and health sciences Minimum inhibitory concentration Drug Resistance Bacterial medicine Escherichia coli Animals Nebramycin Cutoff Escherichia coli Infections 030304 developmental biology Swine Diseases 0303 health sciences lcsh:Veterinary medicine General Veterinary Wild-type cutoff Broth microdilution Wild type 04 agricultural and veterinary sciences General Medicine Anti-Bacterial Agents lcsh:SF600-1100 Research Article medicine.drug
Zdroj:	BMC Veterinary Research, Vol 16, Iss 1, Pp 1-8 (2020) BMC Veterinary Research
ISSN:	1746-6148
DOI:	10.1186/s12917-020-02522-0
Popis:	Background Apramycin is used exclusively for the treatment of Escherichia coli (E.coli) infections in swine around the world since the early 1980s. Recently, many research papers have demonstrated that apramycin has significant in vitro activity against multidrug-resistant E.coli isolated in hospitals. Therefore, ensuring the proper use of apramycin in veterinary clinics is of great significance of public health. The objectives of this study were to develop a wild-type cutoff for apramycin against E.coli using a statistical method recommended by Clinical and Laboratory Standards Institute (CLSI) and to investigate the prevalence of resistance genes that confer resistance to apramycin in E. coli. Results Apramycin susceptibility testing of 1230 E.coli clinical isolates from swine were determinded by broth microdilution testing according to the CLSI document M07-A9. A total number of 310 E.coli strains from different minimum inhibitory concentration (MIC) subsets (0.5–256 μg/mL) were selected for the detection of resistance genes (aac(3)-IV; npmA; apmA) in E. coli by PCR. The percentage of E. coli isolates at each MIC (0.5, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/mL) was 0.08, 0.08, 0.16, 2.93, 31.14, 38.86, 12.85, 2.03, 1.46, and 10.41%. The MIC50 and MIC90 were 16 and 64 μg/mL. All the 310 E.coli isolates were negative for npmA and apmA gene, and only the aac(3)-IV gene was detected in this study. Conclusions The wild-type cutoff for apramycin against E.coli was defined as 32 μg/mL. The prevelance of aac(3)-IV gene mainly concentrated in these MIC subsets ‘MIC ≥ 64 μg/ mL’, which indicates that the wild-type cutoff established in our study is reliable. The wild-type cutoff offers interpretion criteria of apramycin susceptibility testing of E.coli.
Databáze:	OpenAIRE
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