Aminoacid recovery via microdialysis and photoinduced focal cerebral ischemia in brain cortex of rats
| Autor: | V. Rizzo, Lorenza Montalbetti, Enrica Lanza, Roberto Scelsi, Luigia Favalli, Faustino Savoldi, A. Rozza, C. Scavini, G. Racagni |
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| Rok vydání: | 1995 |
| Předmět: |
Male
Pathology medicine.medical_specialty Microdialysis Photochemistry Taurine Ischemia Glutamic Acid Apoptosis Brain Ischemia Brain ischemia Cortex (anatomy) medicine Animals Amino Acids Rats Wistar Cerebral Cortex Neurotransmitter Agents Rose Bengal business.industry General Neuroscience Penumbra Glutamate receptor Reproducibility of Results medicine.disease Rats Disease Models Animal medicine.anatomical_structure Cerebral cortex Neuron business Neuroscience |
| Zdroj: | Neuroscience Letters. 192:153-156 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/0304-3940(95)11632-7 |
| Popis: | A photochemical method using the Rose Bengal dye as thrombogenic agent was employed to induce focal cerebral ischemia in frontoparietal cortex of rats. A transcerebral microdialysis probe was used to collect samples from ischemic cortical area. An increase in glutamate (6-fold) and in taurine (4-fold) within the first hour occurred. Neuropathological investigations demonstrate a reproducible damaged area surrounded by a thin peripheral area showing neuronal apoptotic phenomena. The method represents a reproducible model of focal cerebral ischemia with neuropathological aspects superimposable to those characteristic of thrombogenic stroke in man. This method could also be relevant in the study of neurotransmitters during the evolution of ischemia. Furthermore, the presence of apoptotic phenomena in the perilesional halo confirms an ischemic penumbra suggesting the significance of preclinical pharmacological trials. |
| Databáze: | OpenAIRE |
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