Callus culture as a new approach for the production of high added value compounds in Ilex paraguariensis: genotype influence, medium optimization and compounds identification
| Autor: | Cícero Deschamps, Juliana Degenhardt-Goldbach, Erik Nunes Gomes, Jéssica de Cássia Tomasi, Fabrício Augusto Hansel, Trong D. Tran, R. L. Grunennvaldt, Peter Brooks |
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| Přispěvatelé: | RENATA LÚCIA GRUNENNVALDT, UFPR, JULIANA DEGENHARDT, CNPF, PETER BROOKS, University of The Sunshine Coast, JÉSSICA DE CÁSSIA TOMASI, UFPR, FABRICIO AUGUSTO HANSEL, CNPF, TRONG TRAN, University of The Sunshine Coast, ERIK N. GOMES, The State University of New Jersey, CÍCERO DESCHAMPS, UFPR. |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Atividade antioxidante Acúmulo de biomassa Erva mate Sucrose Genotype Science antioxidant activity Ilex Paraguariensis biomass accumulation 01 natural sciences Sacarose chemistry.chemical_compound food Chlorogenic acid Ilex paraguariensis 010608 biotechnology Yerba-mate plant growth regulators Multidisciplinary Neochlorogenic acid Plant Extracts fungi food and beverages sucrose South America Regulador de Crescimento food.food Plant Leaves Horticulture chemistry Callus Cytokinin Zeatin yerba mate 010606 plant biology & botany Explant culture |
| Zdroj: | Anais da Academia Brasileira de Ciências, Volume: 92, Issue: 3, Article number: e20181251, Published: 11 NOV 2020 Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA Anais da Academia Brasileira de Ciências, Vol 92, Iss 3 (2020) Anais da Academia Brasileira de Ciências v.92 n.3 2020 Anais da Academia Brasileira de Ciências Academia Brasileira de Ciências (ABC) instacron:ABC |
| ISSN: | 1678-2690 0001-3765 |
| DOI: | 10.1590/0001-3765202020181251 |
| Popis: | Ilex paraguariensis (yerba mate) is a native species from South America and is a rich source of bioactive compounds. There is a lack of research efforts on the phytochemical investigation of callus culture from this species. In the present study, an effort was made to optimize callus culture conditions and to identify secondary compounds. Calli were induced from 10 genotypes using leaf explants and the best genotype was selected to evaluate the effects of cytokinin types and concentrations on callus induction and biomass accumulation. The best genotype and cytokinin treatment were used to conduct one last experiment with sucrose concentrations in culture media and its effects on calli biomass, antioxidant activity and secondary compounds accumulation. Callus initiation was genotype dependent, and the 6-156-6 line had the best response. Zeatin supplemented medium showed higher callus induction rate (82%) and higher biomass accumulation after 120 days (328.2 mg). Higher biomass and secondary compounds accumulation were observed for calli on 3% sucrose medium. Antioxidant activity was not affected by sucrose concentrations. Yerba mate callus culture allowed the accumulation of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, theobromine and caffeine. Made available in DSpace on 2020-11-12T19:28:38Z (GMT). No. of bitstreams: 1 Juliana-Grunennvaldt-et-al-2020.pdf: 2458046 bytes, checksum: 2b9d4b4ac4490ff775e3d409a43f475a (MD5) Previous issue date: 2020 |
| Databáze: | OpenAIRE |
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