Proteomics reveals major components of oogenesis in the reproductive tract of sugar-fed Anopheles aquasalis
| Autor: | Andre Borges-Veloso, Geovane Dias-Lopes, Patricia Cuervo, Gabriel Padrón, Cássia Luana de Faria Castro, Leonardo Saboia-Vahia, Constança Britto, Ana Carolina Ramos Guimarães, Jose Batista de Jesus |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Proteomics 0301 basic medicine 030231 tropical medicine Carbohydrates Oogenesis 03 medical and health sciences Vitellogenin 0302 clinical medicine Anopheles Animals Gene Serine protease Regulation of gene expression General Veterinary biology Reproduction General Medicine biology.organism_classification Molecular biology Cell biology 030104 developmental biology Infectious Diseases Gene Expression Regulation Insect Science biology.protein Female Parasitology Vitellogenins |
| Zdroj: | Parasitology Research. 115:1977-1989 |
| ISSN: | 1432-1955 0932-0113 |
| Popis: | Anopheles (Nyssorhynchus) aquasalis is a malaria vector mainly distributed along the coastal regions of South and Central America. In the absence of an effective vaccine against malaria, strategies for controlling the vector are the main tool for interrupting parasite transmission. Mechanisms of oogenesis and embryogenesis in anautogenous mosquitoes are mainly modulated by blood feeding. However, the expression, at the protein level, of genes involved in such mechanisms in sugar-fed females is unknown. In this work, total protein extracts of the reproductive tract of female An. aquasalis that were fed sugar were analyzed using liquid chromatography followed by mass spectrometry for protein identification and bioinformatic tools for data mining. We identified 922 proteins expressed in the organ, and using several databases, we attributed biological meaning for several of them. Remarkably, nine proteins involved in oogenesis were identified in females fed sugar. Putative vitellogenins, vitellogenin receptor, lipid storage droplet, transferrin, ferritin, and apolipoprotein, identified here, are proteins involved in egg development. Proteins involved in embryonic development, such as paxillin, exuperantia, several growth factors, and dorsal switch protein, were identified. Interestingly, in this study, we identified 15 peptidases of various classes such as aminopeptidases, carboxypeptidases, serine protease, cathepsin, and metalloprotease that could potentially interact with male seminal components. Here, we demonstrated that the reproductive tract of female An. aquasalis fed on sugar expresses proteins involved in oogenesis and embryonic development. These findings reveal unknown aspects of the physiology of this organ under the given nutritional conditions. |
| Databáze: | OpenAIRE |
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