Validation of a minimal panel of antibodies for the diagnosis of malignant pleural mesothelioma
| Autor: | Nicola J. Armstrong, Janette L. Vardy, Nico van Zandwijk, Kenneth Lee, Steven Kao, Kim M. Griggs, Stephen Clarke, Sonja Klebe, Brian C. McCaughan, Douglas W. Henderson, Juliet Burn |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Mesothelioma Oncology medicine.medical_specialty Pathology Lung Neoplasms Pleural Neoplasms Lobular carcinoma Breast Neoplasms CD15 Adenocarcinoma Sensitivity and Specificity Pathology and Forensic Medicine Metastatic carcinoma Internal medicine medicine Humans Lung biology business.industry medicine.disease Immunohistochemistry Serous fluid medicine.anatomical_structure biology.protein Pleura Female Calretinin Antibody business |
| Zdroj: | Pathology. 43:313-317 |
| ISSN: | 0031-3025 |
| Popis: | Summary Aims We previously established the use of a minimal panel of antibodies as sufficient to diagnose most epithelial malignant mesothelioma (MPM). We aimed to validate this approach and investigate the utility of a D2-40 antibody. Methods A series of 80 MPM patients selected for surgery and 21 consecutive patients with pleural metastatic carcinoma were included. A minimal panel of antibodies, consisting of calretinin, BG8 and CD15, and D2-40 was investigated. Results Therewere61 epithelial and 19 biphasic MPM as well as 12 metastatic lung, six breast (5 ductal adenocarcinomas, 1 mixed ductal/lobular adenocarcinoma), two serous papillary ovarian carcinomas and one moderately differentiated colorectal adenocarcinoma. The sensitivity of positive calretinin labelling to confirm the diagnosis of MPM was 97.5%, while the ‘diagnostic sensitivities’ of lack of labelling for BG8 and CD15 were 91.3% and 97.5%, respectively. The use of calretinin, BG8 and CD15 resulted in correct classification in 97.5% of all MPMs. All MPM cases investigated showed at least focal positive D2-40 labelling. Conclusions We have validated the usefulness of a minimal panel of antibodies with calretinin, BG8and CD15as the initial step to the diagnosis of MPM. D2-40 emerged as a helpful diagnostic tool for cases where our initial approach failed to conclusively diagnose MPM. |
| Databáze: | OpenAIRE |
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