Molecular cloning of two pigment-dispersing hormone (PDH) precursors in the blue crab Callinectes sapidus reveals a novel member of the PDH neuropeptide family
| Autor: | J.M. Klein, K.R. Rao, C.J. Mohrherr, F. Sleutels, J.P. Riehm |
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| Rok vydání: | 1994 |
| Předmět: |
clone (Java method)
animal structures Callinectes DNA Complementary Brachyura Molecular Sequence Data Biophysics Molecular cloning Biochemistry Animals Carcinus maenas Amino Acid Sequence Cloning Molecular Protein Precursors Molecular Biology Peptide sequence biology Base Sequence Sequence Homology Amino Acid cDNA library Neuropeptides Cell Biology Anatomy biology.organism_classification Eyestalk Open reading frame Peptides |
| Zdroj: | Biochemical and biophysical research communications. 205(1) |
| ISSN: | 0006-291X |
| Popis: | A cDNA library was established from the eyestalk ganglia of the blue crab Callinectes sapidus. Screening resulted in the isolation of a clone (497 bp excluding poly(A) tail) which encodes a beta-PDH previously found in several crustacean species. It displays high sequence similarity with a clone isolated from an eyestalk cDNA library of the shore crab Carcinus maenas, indicating the close phylogenetic relationship of both species. A second clone (414 bp exclusive of the poly(A) tail) encodes a novel beta-PDH analog which displays 400-fold less potency in crab bioassays. Both cDNAs encode open reading frames of 234 bp for the prepropeptides, consisting of signal peptides, PDH-precursor-related peptides, and PDH sequences. |
| Databáze: | OpenAIRE |
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