A rapid, simple and sensitive liquid chromatography tandem mass spectrometry assay to determine amoxicillin concentrations in biological matrix of little volume

Autor: Fiordiligie Casilag, Charlotte Kloft, Robin Michelet, Jan Felix Joseph, Jean-Claude Sirard, Sebastian G. Wicha, Tania Fuhrmann-Selter, Sebastian Franck
Přispěvatelé: Institut für Pharmazie, Freie Universität Berlin, Freie Universität Berlin, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Sirard, Jean-Claude, Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Electrospray
Formic acid
Bioanalytical method validation
02 engineering and technology
01 natural sciences
Mouse serum
Analytical Chemistry
Matrix (chemical analysis)
Mice
chemistry.chemical_compound
Limit of Detection
Tandem Mass Spectrometry
Liquid chromatography–mass spectrometry
medicine
Animals
Protein precipitation
Sample preparation
Pharmacokinetics
LC-MS/MS
Chromatography
[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology
medicine.diagnostic_test
Chemistry
010401 analytical chemistry
Selected reaction monitoring
Amoxicillin
[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences
021001 nanoscience & nanotechnology
0104 chemical sciences
3. Good health
[SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciences
Therapeutic drug monitoring
Calibration
0210 nano-technology
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Chromatography
Liquid
Zdroj: Talanta
Talanta, 2019, 201, pp.253-258. ⟨10.1016/j.talanta.2019.03.098⟩
Talanta, Elsevier, 2019, 201, pp.253-258. ⟨10.1016/j.talanta.2019.03.098⟩
ISSN: 0039-9140
DOI: 10.1016/j.talanta.2019.03.098⟩
Popis: International audience; To assess pharmacokinetics of amoxicillin (AMX) in mice, limitations such as a small sampling volume and low drug concentrations have to be addressed. Similar challenges are faced in a clinical framework, e.g. for therapeutic drug monitoring in neonates or small-scale in vitro investigations. An assay enabling quantification of small sample volumes but still at very low concentrations covering a broad concentration range is thus needed. A simple, rapid and highly sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and successfully validated for quantification of AMX in mouse serum according to European Medicines Agency guidelines. Sample preparation enabled the use of only 10 μL of serum, which is 5-fold less than comparable assays and allows to reduce the number of mice used in pharmacokinetic studies. After protein precipitation with 40 μL chilled methanol and dilution of the supernatant with water, the sample was injected into the LC system on a Poroshell 120 Phenyl Hexyl column (2.1 × 100 mm, 2.7 μm). Chromatographic separation was achieved using a gradient method consisting of acetonitrile and ultra-pure water, both with 0.1% (V/V) formic acid. Positive electrospray ionisation in multiple reaction monitoring mode was used for detection and quantification of AMX. Application to murine study samples demonstrated the reliability of the developed method being accurate and precise with a quantification range from 0.01 to 10 μg/mL. The assay is easily transferable due to a simple sample preparation and confirmed stability of AMX under various applied conditions.
Databáze: OpenAIRE
Externí odkaz: