OatA, a Peptidoglycan O-Acetyltransferase Involved in Listeria monocytogenes Immune Escape, Is Critical for Virulence
| Autor: | Martin Sachse, Pascale Cossart, Olivier Dussurget, Jérémie Decalf, Céline Goulard, Ivo G. Boneca, Camille Aubry, Marie-Anne Nahori, Nadège Cayet |
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| Přispěvatelé: | Interactions Bactéries-Cellules (UIBC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Recherche Agronomique (INRA), Microscopie ultrastructurale (plate-forme), Institut Pasteur [Paris], Immunobiologie des Cellules Dendritiques, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Groupe Avenir, Institut National de la Santé et de la Recherche Médicale (INSERM), Biologie et Génétique de la Paroi bactérienne - Biology and Genetics of Bacterial Cell Wall (BGPB), Université Paris Diderot - Paris 7 (UPD7), This work was supported by Institut Pasteur, Institut National de la Santé et de la Recherche Médicale, Institut National de la Recherche Agronomique, European Research Council (Advanced grant 233348 to P. C. and Starting grant 202283 to I. G. B.), Fondation Pasteur-Weizmann, Fondation Jeantet, and Fondation le Roch Les Mousquetaires. P. C. is an international research scholar of the Howard Hughes Medical Institute. C. A. is a doctoral fellow of the Ministère de l’Enseignement Supérieur et de la Recherche., Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
INTERLEUKIN-6-DEFICIENT MICE
MESH: Liver/microbiology MESH: Spleen/microbiology Mutant MESH: Acetyltransferases/genetics medicine.disease_cause MESH: Acetyltransferases/immunology chemistry.chemical_compound Mice INFECTION Immunology and Allergy Listeriosis MESH: Animals MESH: Virulence Factors/immunology NEUTROPHILS TUMOR-NECROSIS-FACTOR 0303 health sciences Mice Inbred BALB C MESH: Microbial Sensitivity Tests MESH: Th2 Cells/metabolism IFN-GAMMA biology MESH: Macrophages/immunology Acetylation MESH: Listeriosis/immunology 3. Good health Infectious Diseases Liver MESH: Listeria monocytogenes/genetics Muramic Acids Cytokines [SDV.IMM]Life Sciences [q-bio]/Immunology Female MESH: Immunity Innate MESH: Acetylation INTERFERON Virulence Factors MESH: Mice Inbred BALB C Virulence Microbial Sensitivity Tests Peptidoglycan Microbiology Cell Line Lethal Dose 50 03 medical and health sciences Major Articles and Brief Reports Immune system DEFICIENT Th2 Cells Listeria monocytogenes Acetyltransferases MESH: Liver/metabolism MESH: Listeriosis/genetics medicine MESH: Virulence Factors/genetics Animals Humans Secretion MESH: Macrophages/metabolism MESH: Mice MESH: Muramic Acids/metabolism 030304 developmental biology STAPHYLOCOCCUS-AUREUS Innate immune system MESH: Humans MESH: Peptidoglycan/immunology 030306 microbiology MESH: Listeria monocytogenes/growth & development Macrophages MESH: Listeria monocytogenes/pathogenicity Th1 Cells biology.organism_classification Immunity Innate MESH: Cell Line MESH: Listeria monocytogenes/immunology MESH: Lethal Dose 50 chemistry MESH: Th1 Cells/metabolism Listeria MESH: Cytokines/metabolism MESH: Peptidoglycan/chemistry MESH: Female RESISTANCE Spleen RESPONSES |
| Zdroj: | Journal of Infectious Diseases Journal of Infectious Diseases, Oxford University Press (OUP), 2011, 204 (5), pp.731-40. ⟨10.1093/infdis/jir396⟩ Journal of Infectious Diseases, 2011, 204 (5), pp.731-40. ⟨10.1093/infdis/jir396⟩ |
| ISSN: | 0022-1899 1537-6613 |
| DOI: | 10.1093/infdis/jir396⟩ |
| Popis: | International audience; Microbial pathogens have evolved mechanisms to overcome immune responses and successfully infect their host. Here, we studied how Listeria monocytogenes evades immune detection by peptidoglycan (PGN) modification. By analyzing L. monocytogenes muropeptides, we detected O-acetylated muramic acid residues. We identified an O-acetyltransferase gene, oatA, in the L. monocytogenes genome sequence. Comparison of PGN from parental and isogenic oatA mutant strains showed that the O-acetyltransferase OatA O-acetylates Listeria PGN. We also found that PGN O-acetylation confers resistance to different types of antimicrobial compounds targeting bacterial cell wall such as lysozyme, β-lactam antibiotics, and bacteriocins and that O-acetylation is required for Listeria growth in macrophages. Moreover, oatA mutant virulence is drastically affected in mice following intravenous or oral inoculation. In addition, the oatA mutant induced early secretion of proinflammatory cytokines and chemokines in vivo. These results suggest an important role for OatA in limiting innate immune responses and promoting bacterial survival in the infected host. |
| Databáze: | OpenAIRE |
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