Green synthesis of silver nanoparticles using Citrus limon peels and evaluation of their antibacterial and cytotoxic properties
| Autor: | Neveen Abdel Raouf, Nouf M. Al-Enazi, Ashraf A. Hatamleh, Noura S. Aldosari, Jamilah H. Alshehri, Manal M. Alkhulaifi, Manal A. Awad, Moudi Alwehaibi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Green Silver nanoparticles 01 natural sciences Silver nanoparticle 03 medical and health sciences Breast cancer Carcinoma cell line Viability assay Fourier transform infrared spectroscopy IC50 lcsh:QH301-705.5 chemistry.chemical_classification Citrus limon peels Cytotoxic activity Chemistry Antimicrobial Amino acid 030104 developmental biology lcsh:Biology (General) Cell culture Original Article Antibacterial activity General Agricultural and Biological Sciences 010606 plant biology & botany Nuclear chemistry |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 27, Iss 12, Pp 3434-3441 (2020) Saudi Journal of Biological Sciences |
| Popis: | The present work aimed to synthesis silver nanoparticles (AgNPs) using biological waste products Citrus limon peels, its characterization, antimicrobial activities and the cytotoxic effect of the synthesized green AgNPs. Characterization of the prepared AgNPs showed the formation of spherical, and few agglomerated AgNPs forms as measured by UV–visible spectrophotometer. The average size of the prepared AgNPs was 59.74 nm as measured by DLS technique. The spectrum of the synthesized AgNPs was observed at 3 KeV using the EDX. On the other hand, FTIR analysis of the green synthesized AgNPs showed the presence of alcohols, phenolics, mono-substituted alkynes, aliphatic primary amines, sodium salt, amino acid, or SiOH alcohol groups. The antimicrobial studies of the formed AgNPs showed positive activity against most of the studied human pathogenic bacteria with varying degrees. Finally, the evaluation of the cytotoxic effect of the green synthesized AgNPs were done using two types of cell lines, human breast cancer cell line (MCF-7) and human colon carcinoma cell line (HCT-116). The results revealed the concentration has a direct correlation with cell viability. The 50% inhibitory concentration (IC50) of MCF-7 cell line was in of 23.5 ± 0.97 µL/100 µL, whereas the HCT-116 cell line was in 37.48 ± 5.93 µL/100 µL. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|