High-refined carbohydrate diet consumption induces neuroinflammation and anxiety-like behavior in mice
| Autor: | Anna Paula Marçal, Giovanni Freitas Gomes, Josiane F. Silva, Marina C. Oliveira, Antônio Carlos Pinheiro de Oliveira, Julia A.S. Gomes, Adaliene Versiani Matos Ferreira, Grazielle C. Silva, Daniele C. Aguiar, Virginia L. Soares |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Leptin Male medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Adipose tissue Nitric Oxide Synthase Type II Prefrontal Cortex Inflammation Anxiety Biochemistry Guanidines Hippocampus Nitric oxide 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine Internal medicine medicine Dietary Carbohydrates Animals Molecular Biology Neuroinflammation Nitrites Mice Inbred BALB C Nutrition and Dietetics biology Behavior Animal Chemistry Nitric oxide synthase Disease Models Animal 030104 developmental biology Endocrinology Cytokine Adipose Tissue biology.protein Tumor necrosis factor alpha medicine.symptom 030217 neurology & neurosurgery |
| Zdroj: | The Journal of nutritional biochemistry. 77 |
| ISSN: | 1873-4847 |
| Popis: | Consumption of poor nutrients diets is associated with fat tissue expansion and with a central and peripheral low-grade inflammation. In this sense, the microglial cells in the central nervous system are activated and release pro-inflammatory cytokines that up-regulate the inducible nitric oxide synthase (iNOS), promoting Nitric Oxide (NO) production. The excess of NO has been proposed to facilitate anxious states in humans and rodents. We evaluated whether consumption of a high-refined carbohydrate-containing diet (HC) in mice induced anxiety-like behavior in the Novelty Suppressed Feeding Test (NFST) trough facilitation of NO, in the prefrontal cortex (PFC) and hippocampus (HIP). We also verified if HC diet induces activation of microglial cells, alterations in cytokine and leptin levels in such regions. Male BALB/c mice received a standard diet or a HC diet for 3 days or 12 weeks. The chronic consumption of HC diet, but not acute, induced an anxiogenic-like effect in the NSF test and an increase in the nitrite levels in the PFC and HIP. The preferential iNOS inhibitor, aminoguanidine (50 mg/kg, i.p.), attenuated such effects. Moreover, microglial cells in the HIP and PFC were activated after chronic consumption of HC diet. Finally, the expression of iNOS in the PFC and TNF, IL6 and leptin levels in HIP were higher in chronically HC fed mice. Taken together, our data reinforce the notion that diets containing high-refined carbohydrate facilitate anxiety-like behavior, mainly after a long period of consumption. The mechanisms involve, at least in part, the augmentation of neuroinflammatory processes in brain areas responsible for anxiety control. |
| Databáze: | OpenAIRE |
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