Nasopharyngeal brushing: a convenient and feasible sampling method for nucleic acid-based nasopharyngeal carcinoma research

Autor: Shao Dan Zhang, Xi Zhao Li, Ye Zhu Hu, Tian Tian, Wei Hua Jia, Pei Fen Zhang, Xiao Hui Zheng
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Viral nucleic acid
medicine.medical_specialty
Epstein-Barr Virus Infections
Herpesvirus 4
Human
Cancer research
Tissue samples
Gastroenterology
lcsh:RC254-282
Virus
Specimen Handling
03 medical and health sciences
0302 clinical medicine
Internal medicine
Nasopharynx
otorhinolaryngologic diseases
medicine
Nasopharyngeal carcinoma
Humans
business.industry
Endoscopic biopsy
Nasopharyngitis
Reproducibility of Results
Endoscopy
Nasopharyngeal Neoplasms
DNA
Neoplasm
medicine.disease
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
MicroRNAs
stomatognathic diseases
030104 developmental biology
Real-time polymerase chain reaction
Oncology
Nucleic acid
030220 oncology & carcinogenesis
Healthy individuals
DNA
Viral
Feasibility Studies
RNA
Viral
business
Nasopharyngeal brushing
Zdroj: Cancer Communications, Vol 38, Iss 1, Pp 1-10 (2018)
ISSN: 2523-3548
Popis: Background Tissue specimens for nasopharyngeal carcinoma (NPC) research are scarce because of sampling difficulties. Previous studies have suggested non-invasive nasopharyngeal brushing as an effective sampling method for NPC diagnosis. The present study aimed to evaluate the feasibility of nasopharyngeal brushing in the acquisition of NPC nucleic acids for research. Methods Nasopharyngeal brushing samples were acquired from 24 healthy individuals and 48 NPC patients. Tissues from 48 NPC and 18 nasopharyngitis patients were collected by endoscopic biopsy. The expression levels of tumor suppressor genes (TSGs) and Epstein–Barr virus (EBV)-encoded microRNAs as well as EBV DNA copy number were measured by quantitative polymerase chain reaction in both types of samples. Results Among six TSGs examined, the expression levels of two genes were significantly decreased in nasopharyngeal brushing and tissue samples from NPC patients as compared with those from healthy/nasopharyngitis individuals. Four EBV-encoded microRNAs, mir-bart1-5p, mir-bart5, mir-bart6-5p, and mir-bart17-5p, were significantly up-regulated in both NPC brushing and tissue samples compared with those from healthy/nasopharyngitis controls (P
Databáze: OpenAIRE
Externí odkaz: