Nasopharyngeal brushing: a convenient and feasible sampling method for nucleic acid-based nasopharyngeal carcinoma research
Autor: | Shao Dan Zhang, Xi Zhao Li, Ye Zhu Hu, Tian Tian, Wei Hua Jia, Pei Fen Zhang, Xiao Hui Zheng |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Viral nucleic acid medicine.medical_specialty Epstein-Barr Virus Infections Herpesvirus 4 Human Cancer research Tissue samples Gastroenterology lcsh:RC254-282 Virus Specimen Handling 03 medical and health sciences 0302 clinical medicine Internal medicine Nasopharynx otorhinolaryngologic diseases medicine Nasopharyngeal carcinoma Humans business.industry Endoscopic biopsy Nasopharyngitis Reproducibility of Results Endoscopy Nasopharyngeal Neoplasms DNA Neoplasm medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens MicroRNAs stomatognathic diseases 030104 developmental biology Real-time polymerase chain reaction Oncology Nucleic acid 030220 oncology & carcinogenesis Healthy individuals DNA Viral Feasibility Studies RNA Viral business Nasopharyngeal brushing |
Zdroj: | Cancer Communications, Vol 38, Iss 1, Pp 1-10 (2018) |
ISSN: | 2523-3548 |
Popis: | Background Tissue specimens for nasopharyngeal carcinoma (NPC) research are scarce because of sampling difficulties. Previous studies have suggested non-invasive nasopharyngeal brushing as an effective sampling method for NPC diagnosis. The present study aimed to evaluate the feasibility of nasopharyngeal brushing in the acquisition of NPC nucleic acids for research. Methods Nasopharyngeal brushing samples were acquired from 24 healthy individuals and 48 NPC patients. Tissues from 48 NPC and 18 nasopharyngitis patients were collected by endoscopic biopsy. The expression levels of tumor suppressor genes (TSGs) and Epstein–Barr virus (EBV)-encoded microRNAs as well as EBV DNA copy number were measured by quantitative polymerase chain reaction in both types of samples. Results Among six TSGs examined, the expression levels of two genes were significantly decreased in nasopharyngeal brushing and tissue samples from NPC patients as compared with those from healthy/nasopharyngitis individuals. Four EBV-encoded microRNAs, mir-bart1-5p, mir-bart5, mir-bart6-5p, and mir-bart17-5p, were significantly up-regulated in both NPC brushing and tissue samples compared with those from healthy/nasopharyngitis controls (P |
Databáze: | OpenAIRE |
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