Leukotriene C4 biosynthesis in isolated August rat peritoneal leukocytes
| Autor: | Robert R. Eversole, W. F. Jackson, S. R. Stapleton, Charles D. Mackenzie, J. M. Huebner, L. J. Beuving |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Leukotriene
education.field_of_study Leukotriene C4 business.industry Immunology Population Cell Biology Microbiology chemistry.chemical_compound medicine.anatomical_structure chemistry Peritoneum Biosynthesis medicine lcsh:Pathology Eosinophilia medicine.symptom education business Protein kinase C Research Article Isolated cell lcsh:RB1-214 |
| Zdroj: | Mediators of Inflammation, Vol 5, Iss 6, Pp 443-447 (1996) Mediators of Inflammation |
| ISSN: | 0962-9351 |
| DOI: | 10.1155/S0962935196000610 |
| Popis: | The mixed leukocyte population obtained from the peritoneum of the August rat is a potentially important experimental model of inherent eosinophilia that has not been well characterized. In the present study, isolated cell preparations generated a concentration-dependent release of leukotriene (LT) C4when exposed to the Ca2+ionophore A23187, reaching maximal stimulation at 5.0 μM. This response was inhibited by the 5-lipoxygenase activating protein antagonist MK-886 (0.1 μM), nominally Ca2+and Mg2+-free incubation media and by activation of protein kinase C via phorbol 12-myristate 13-acetate (50 nM). These findings establish a model system for investigating LTC4profiles contingent with innate peritoneal eosinophilia and are consistent with the hypothesis that cellular LTC4biosynthesis is phosphoregulated. |
| Databáze: | OpenAIRE |
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