Molecular mimicry in Campylobacter jejuni: role of the lipo-oligosaccharide core oligosaccharide in inducing anti-ganglioside antibodies
| Autor: | Peter J. Coloe, Malcolm J. McConville, John H. Patterson, Irving Nachamkin, Benjamin N. Fry, Viraj N. Perera, Huong Ung |
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| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
medicine.drug_class Virulence Factors Immunology Molecular Sequence Data Oligosaccharides medicine.disease_cause Monoclonal antibody Microbiology Campylobacter jejuni Polymerase Chain Reaction Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Mice Antigen Gangliosides Campylobacter Infections medicine Immunology and Allergy Animals Amino Acid Sequence Cloning Molecular Antigens Bacterial Mice Inbred C3H Ganglioside biology Cholera toxin Molecular Mimicry General Medicine biology.organism_classification Antibodies Bacterial Sialic acid Molecular mimicry Infectious Diseases chemistry Glucosyltransferases biology.protein Immunization Antibody |
| Zdroj: | FEMS immunology and medical microbiology. 50(1) |
| ISSN: | 0928-8244 |
| Popis: | Campylobacter jejuni is recognized as the most common identifiable pathogen associated with the development of Guillain-Barre syndrome (GBS), an acute autoimmune-mediated disease affecting the peripheral nervous system. The immune response to ganglioside-like structures in lipo-oligosaccharides (LOSs) of certain C. jejuni strains is thought to cross-react with human nerve gangliosides and induce GBS. To study the involvement of LOSs in the pathogenesis of Campylobacter-induced GBS, we created truncated LOS molecules by inactivating the waaF gene in a GBS-associated isolate of C. jejuni. Gas Chromatography-MS analysis of the waaF mutant LOSs revealed a marked reduction in sugar content, including sialic acid and galactose. GM1 and GD1a-like mimicry was not detected in the waaF mutant by Western blot analysis with cholera toxin B and anti-GD1a antibodies. Mice immunized with the waaF mutant failed to develop anti-GM1 or anti-GD1a antibodies. The waaF mutant also showed reduced adherence to and invasion of INT-407 cells. The results indicate that the LOS of C. jejuni HB93-13 is essential for adherence and invasion as well as for anti-ganglioside antibody induction. |
| Databáze: | OpenAIRE |
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