Functional complementation of a membrane transport deficiency in Saccharomyces cerevisiae by recombinant ND4 fusion protein
| Autor: | Michael J. Ellison, Douglas L. Hogue, Mark F. Vickers, Carol E. Cass |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Vesicle-associated membrane protein 8 DNA Complementary Recombinant Fusion Proteins Saccharomyces cerevisiae Molecular Sequence Data Biophysics Respiratory chain Biology Biochemistry Fungal Proteins Humans Amino Acid Sequence RNA Messenger Molecular Biology Integral membrane protein Base Sequence Cell Membrane Genetic Complementation Test nutritional and metabolic diseases Galactose Biological Transport NADH Dehydrogenase Cell Biology Membrane transport biology.organism_classification Fusion protein eye diseases Transport protein Transmembrane domain |
| Zdroj: | Biochemical and biophysical research communications. 238(3) |
| ISSN: | 0006-291X |
| Popis: | ND4 is a mitochondrially encoded component of NADH dehydrogenase (Complex I) of the respiratory chain. A cDNA encoding a fusion protein comprised of the N-terminus of GAL10 of Saccharomyces cerevisiae and an N-terminally truncated form of human ND4 was isolated by its ability to functionally complement the thymidine transport deficiency of S. cerevisiae grown under conditions of thymidylate starvation. Epitope-tagged GAL10 -ND4 was shown by immunological methods to be present in the plasma membranes of yeast expressing the GAL10 -ND4 encoding cDNA. The ability of the GAL10 -ND4 fusion protein to induce uptake of thymidine raises the possibility that native ND4, which is predicted to have 12 transmembrane domains, may function as a transporter or channel in the mitochondrial inner membrane. |
| Databáze: | OpenAIRE |
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