Virucidal and antiviral activities of pomegranate (Punica granatum) extract against the mosquito-borne Mayaro virus
| Autor: | Marcelo Damião Ferreira de Meneses, Renata Campos Azevedo, Tiago Souza Salles, Ricardo Machado Kuster, José A. Ventura, Márcia Regina Soares, Danielle M. de Oliveira, Davis Fernandes Ferreira, Lucio Ayres Caldas, Thayane da Encarnação Sá-Guimarães |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
viruses
Phytochemicals Infectious and parasitic diseases RC109-216 Alphavirus Virus Replication Antiviral Agents Virus Pomegranate chemistry.chemical_compound Chlorocebus aethiops Biflavonoids Animals Cytotoxicity Vero Cells Punicalagin biology Traditional medicine Research biology.organism_classification Mayaro virus Hydrolyzable Tannins Infectious Diseases Culicidae Parasitology chemistry Phytochemical Punica Vero cell Arboviruses |
| Zdroj: | Parasites & Vectors Parasites & Vectors, Vol 14, Iss 1, Pp 1-8 (2021) |
| ISSN: | 1756-3305 |
| Popis: | Background The arthropod-borne Mayaro virus (MAYV) causes “Mayaro fever,” a disease of medical significance, primarily affecting individuals in permanent contact with forested areas in tropical South America. Recently, MAYV has attracted attention due to its likely urbanization. There are currently no licensed drugs against most mosquito-transmitted viruses. Punica granatum (pomegranate) fruits cultivated in Brazil have been subjected to phytochemical investigation for the identification and isolation of antiviral compounds. In the present study, we explored the antiviral activity of pomegranate extracts in Vero cells infected with Mayaro virus. Methods The ethanol extract and punicalagin of pomegranate were extracted solely from the shell and purified by chromatographic fractionation, and were chemically identified using spectroscopic techniques. The cytotoxicity of the purified compounds was measured by the dye uptake assay, while their antiviral activity was evaluated by a virus yield inhibition assay. Results Pomegranate ethanol extract (CC50 = 588.9, IC50 = 12.3) and a fraction containing punicalagin as major compound (CC50 = 441.5, IC50 = 28.2) were shown to have antiviral activity (SI 49 and 16, respectively) against Mayaro virus, an alphavirus. Immunofluorescence analysis showed the virucidal effect of pomegranate extract, and transmission electron microscopy (TEM) revealed damage in viral particles treated with this extract. Conclusions The P. granatum extract is a promising source of antiviral compounds against the alphavirus MAYV and represents an excellent candidate for future studies with other enveloped RNA viruses. Graphical abstract |
| Databáze: | OpenAIRE |
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