Real-Time, Label-Free Detection of Local Exocytosis Outside Pancreatic β Cells Using Laser Tweezers Raman Spectroscopy
| Autor: | Xi Rong, Rui-qiong Luo, Hong Liu, Yue-ming Jiang, Fang Wei, Shu-shi Huang, Wen-qing Mo, Shan-lei Zhang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Optical Tweezers Analytical chemistry Spectrum Analysis Raman Exocytosis Cell membrane 03 medical and health sciences symbols.namesake 0302 clinical medicine Cell Line Tumor Insulin-Secreting Cells medicine Animals Instrumentation Spectroscopy Chemistry Calcium channel Rats Electrophysiology Microelectrode 030104 developmental biology medicine.anatomical_structure Optical tweezers Biophysics symbols Single-Cell Analysis Raman spectroscopy 030217 neurology & neurosurgery Raman scattering |
| Zdroj: | Applied Spectroscopy. 71:422-431 |
| ISSN: | 1943-3530 0003-7028 |
| DOI: | 10.1177/0003702816670911 |
| Popis: | The examination of insulin (Ins) exocytosis at the single-cell level by conventional methods, such as electrophysiological approaches, total internal reflection imaging, and two-photon imaging technology, often requires an invasive microelectrode puncture or label. In this study, high concentrations of glucose and potassium chloride were used to stimulate β cell Ins exocytosis, while low concentrations of glucose and calcium channel blockers served as the blank and negative control, respectively. Laser tweezers Raman spectroscopy (LTRS) was used to capture the possible Raman scattering signal from a local zone outside of the cell edge. The results show that the frequencies of the strong signals from the local zones outside the cellular edge in the stimulated groups are greater than those of the control. The Raman spectra from the cellular edge, Ins and cell membrane were compared. Thus, local Ins exocytosis activity outside pancreatic β cells might be observed indirectly using LTRS, a non-invasive optical method. |
| Databáze: | OpenAIRE |
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