Force-history dependence and cyclic mechanical reinforcement of actin filaments at the single molecular level
| Autor: | Larry V. McIntire, Shoichiro Ono, Hyunjung Lee, Cheng Zhu, Suzanne G. Eskin |
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| Rok vydání: | 2019 |
| Předmět: |
Genetic Vectors
Kinetics Gene Expression macromolecular substances Biology Microscopy Atomic Force Mechanotransduction Cellular Avian Proteins 03 medical and health sciences 0302 clinical medicine Ultimate tensile strength Escherichia coli Animals Humans Cloning Molecular Mechanotransduction Cytoskeleton Actin 030304 developmental biology CapZ Actin Capping Protein 0303 health sciences Cell Biology Actin cytoskeleton Actins Recombinant Proteins Single Molecule Imaging Actin Cytoskeleton Protein Subunits Biophysics Force dynamics Mechanosensitive channels Stress Mechanical Chickens 030217 neurology & neurosurgery Research Article Protein Binding Tropomodulin |
| Zdroj: | Journal of Cell Science. |
| ISSN: | 1477-9137 0021-9533 |
| Popis: | The actin cytoskeleton is subjected to dynamic mechanical forces over time and the history of force loading may serve as mechanical preconditioning. While the actin cytoskeleton is known to be mechanosensitive, the mechanisms underlying force regulation of actin dynamics still need to be elucidated. Here, we investigated actin depolymerization under a range of dynamic tensile forces using atomic force microscopy. Mechanical loading by cyclic tensile forces induced significantly enhanced bond lifetimes and different force-loading histories resulted in different dissociation kinetics in G-actin–G-actin and G-actin–F-actin interactions. Actin subunits at the two ends of filaments formed bonds with distinct kinetics under dynamic force, with cyclic mechanical reinforcement more effective at the pointed end compared to that at the barbed end. Our data demonstrate force-history dependent reinforcement in actin–actin bonds and polarity of the actin depolymerization kinetics under cyclic tensile forces. These properties of actin may be important clues to understanding regulatory mechanisms underlying actin-dependent mechanotransduction and mechanosensitive cytoskeletal dynamics. This article has an associated First Person interview with the first author of the paper. |
| Databáze: | OpenAIRE |
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