Human brain microvascular endothelial cells resist elongation due to shear stress
| Autor: | Peter C. Searson, Mao Ye, Jackson G. DeStefano, Andrew D. Wong, Adam Reinitz |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Biology
Blood–brain barrier Cell morphology Biochemistry Article Umbilical vein Cell Line Lab-On-A-Chip Devices Human Umbilical Vein Endothelial Cells Shear stress medicine Shear strength Humans Cytoskeleton beta Catenin Actin Cell Proliferation Microcirculation Brain Endothelial Cells Cell Biology Actins Cell biology Phenotype medicine.anatomical_structure Microscopy Fluorescence Blood-Brain Barrier Cell culture Zonula Occludens-1 Protein Stress Mechanical Shear Strength Cardiology and Cardiovascular Medicine Blood Flow Velocity |
| Zdroj: | Microvascular Research. 99:8-18 |
| ISSN: | 0026-2862 |
| DOI: | 10.1016/j.mvr.2015.02.008 |
| Popis: | Endothelial cells in straight sections of vessels are known to elongate and align in the direction of flow. This phenotype has been replicated in confluent monolayers of bovine aortic endothelial cells and human umbilical cord endothelial vein cells (HUVECs) in cell culture under physiological shear stress. Here we report on the morphological response of human brain microvascular endothelial cells (HBMECs) in confluent monolayers in response to shear stress. Using a microfluidic platform we image confluent monolayers of HBMECs and HUVECs under shear stresses up to 16 dyne cm−2. From live-cell imaging we quantitatively analyze the cell morphology and cell speed as a function of time. We show that HBMECs do not undergo a classical transition from cobblestone to spindle-like morphology in response to shear stress. We further show that under shear stress, actin fibers are randomly oriented in the cells indicating that there is no cytoskeletal remodeling. These results suggest that HBMECs are programmed to resist elongation and alignment under shear stress, a phenotype that may be associated with the unique properties of the blood–brain barrier. |
| Databáze: | OpenAIRE |
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