Antioxidant and anti-inflammatory activities ofPittocaulonspecies from México
| Autor: | J. Camilo Marín-Loaiza, Carlos L. Céspedes, Antonio Nieto-Camacho |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
DPPH Thiobarbituric acid Pittocaulon Anti-Inflammatory Agents Pharmaceutical Science Asteraceae Carrageenan Plant Roots Antioxidants Mice chemistry.chemical_compound Drug Discovery Edema Enzyme Inhibitors Plant Stems biology Traditional medicine Chemistry Brain General Medicine Tetradecanoylphorbol Acetate Molecular Medicine Pittocaulon praecox medicine.drug_class Context (language use) Flowers Thiobarbituric Acid Reactive Substances Anti-inflammatory Picrates Botany medicine TBARS Animals Rats Wistar Mexico Peroxidase Pharmacology Methylene Chloride Plants Medicinal Dose-Response Relationship Drug Plant Extracts Methanol Biphenyl Compounds biology.organism_classification Rats Disease Models Animal Complementary and alternative medicine Solvents Lipid Peroxidation |
| Zdroj: | Pharmaceutical Biology. 51:260-266 |
| ISSN: | 1744-5116 1388-0209 |
| DOI: | 10.3109/13880209.2012.718352 |
| Popis: | context: Stems and leaves of Pittocaulon spp. (Asteraceae) are used in Mexican traditional medicine as an anti-inflammatory substance and for the treatment of skin injuries.This study evaluated the antioxidant activity of methanol (MeOH) and dichloromethane (DC) extracts of five Pittocaulon species.DC and MeOH extracts from flowers, roots, and stems of Pittocaulon praecox (Cav.) H. Rob.Brettell, P. bombycophole (Bullock) H. Rob.Brettell, P. filare (Mc Vaugh) H. Rob.Brettell, P. velatum (Greenm.) Rob.Brettell and P. hintonii H. Rob.Brettell.In the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, the flower extracts obtained with MeOH were the most active with IC(50) values ranging from 51.83 ± 4.08 to 154.19 ± 8.39 ppm. In the thiobarbituric acid reactive substances (TBARS) model, the best activity was shown by DC extracts of roots with IC(50) values ranging from 55.54 ± 1.28 to 160.82 ± 5.37 ppm. The MeOH extract of flowers of P. bombycophole had the highest IC(50) value in both DPPH (51.83 ± 4.08 ppm) and TBARS (39.78 ± 1.97 ppm). The samples with the best values in the antioxidant activity assays were evaluated in the anti-inflammatory tests. The DC root extract of P. velatum at a dose of 1 mg/ear produced the greatest reduction (84.96%) of the 2-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema. This extract also reduced the activity of the enzyme myeloperoxidase (MPO) (73.65%) at the same dose. In contrast, DC root extract of this species did not show significant inhibition of the increase in paw edema induced by carrageenan at the doses tested (100 mg/kg).These results support the traditional use of these plants as anti-inflammatory. DC extracts of P. velatum and MeOH extracts of P. bombycophole may be a potential resource of natural anti-inflammatory and antioxidant compounds, respectively. Additional studies must be done to identify the compounds responsible of the activity on these plants and to establish the mechanism of action. |
| Databáze: | OpenAIRE |
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