Beam induced mass loss in high resolution biological microanalysis
| Autor: | Marie Cantino, L. E. Wilkinson, Dale E. Johnson, M. K. Goddard |
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| Rok vydání: | 1986 |
| Předmět: |
Histology
Ranidae Chemistry Muscles Analytical chemistry Bremsstrahlung Serum Albumin Bovine Contamination Microanalysis Salivary Glands Spectral line Rats Pathology and Forensic Medicine law.invention Microscopy Electron Freeze Drying law Radiation damage Animals Electron microscope Beam (structure) Order of magnitude Electron Probe Microanalysis |
| Zdroj: | Journal of Microscopy. 144:317-327 |
| ISSN: | 0022-2720 |
| Popis: | SUMMARY Electron beam induced loss of mass from the organic matrix and from higher Z constituents of biological samples was measured by monitoring bremsstrahlung and peak changes in EDS spectra. When any effects of contamination, extraneous X-rays, beam current drift, specimen drift, and specimen shrinkage were monitored and corrected for, the three types of samples gave consistent and similar results at 296 K. Bremsstrahlung losses averaged 45%, 46% and 50% respectively for muscle homogenate, salivary gland sections and albumin. Sulphur losses average 74%, 72% and 86% for the same three sample types. No other elements suffered significant losses. Dl/e for bremsstrahlung averaged 0·14 C/cm2. Bremsstrahlung loss at 93 K began approximately one order of magnitude higher in dose, and the extent of loss varied. Sulphur losses, however, were greatly reduced at low temperatures. |
| Databáze: | OpenAIRE |
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