Lymphocyte subpopulation profiles and mitogen kinetics in immunological deficiency testing
| Autor: | Donald J. Kuban, Frederick Hecht, Ruta M. Radvany, Mark S. Schubert, Mary Kiamar, Karen Koehler-Carone, Vince Jarzabek |
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| Rok vydání: | 1987 |
| Předmět: |
Cellular immunity
medicine.medical_specialty Lymphocyte Immunology Population Lymphocyte proliferation In Vitro Techniques Lymphocyte Activation T-Lymphocytes Regulatory Internal medicine Immunopathology medicine Humans Immunology and Allergy Lymphocytes education Acquired Immunodeficiency Syndrome education.field_of_study biology Pokeweed mitogen T-Lymphocytes Helper-Inducer T lymphocyte Kinetics medicine.anatomical_structure Endocrinology Concanavalin A biology.protein Mitogens |
| Zdroj: | Journal of Clinical Immunology. 7:312-318 |
| ISSN: | 1573-2592 0271-9142 |
| Popis: | The correlation between lymphocyte proliferative responses to mitogens and T4/T8 ratios was analyzed in a cross section of patients who either were in a high-risk group for HTLV-III infection or fulfilled clinical criteria for acquired immune deficiency syndrome (AIDS). The patient results showed that, correlated with decreased T4/T8 ratios, there was a decrease in mitogen responsiveness first to pokeweed mitogen (PWM), followed by concanavalin A (Con A) and then phytohemagglutinin (PHA). Parallel to this decrease there was a shift toward higher concentrations of mitogens needed for optimal proliferation. In comparison, depletion of T4+ lymphocytes from normal healthy controls also decreased lymphocyte proliferative responses to all three mitogens but shifted the amount of mitogen needed for optimal proliferation toward lower concentrations. The differences in mitogen-induced proliferation between patients and healthy controls suggest a model whereby there is a functional defect(s) in mitogen responsiveness of the remaining T4- lymphocyte population that can be overcome when higher concentrations of mitogen are used. |
| Databáze: | OpenAIRE |
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