Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats
| Autor: | Hamid Deldar, İrfan Öztürk, Reza Kamaledini, Amir Hossein Abazari-Kia, Nasser Ghanem, Abdollah Mohammadi-Sangcheshmeh, Arash Veshkini, Elmira Mottaghi, Eduardo L. Gastal |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
food.ingredient
medicine.medical_treatment embryo royal jelly Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine food Royal jelly medicine Blastocyst oocyte 030219 obstetrics & reproductive medicine In vitro fertilisation General Veterinary urogenital system Chemistry Embryogenesis 0402 animal and dairy science Embryo 04 agricultural and veterinary sciences Glutathione Oocyte 040201 dairy & animal science In vitro maturation medicine.anatomical_structure embryonic structures gene expression Original Article Animal Science and Zoology in vitro fertilization |
| Zdroj: | Animal Reproduction |
| ISSN: | 1984-3143 |
| DOI: | 10.21451/1984-3143-2017-ar986 |
| Popis: | Royal jelly (RJ) was supplemented to goat oocyte in vitro maturation (IVM) medium at three different concentrations (2.5, 5, and 10 mg/ml). Maturation rate, embryo cleavage, and blastocyst rate were recorded. Gene expression of apoptosis-related transcripts was investigated in matured oocytes. Percentage of oocytes that reached MII-stage was increased in RJ-treated groups compared to the control group. Glutathione (GSH) content of mature oocytes was enhanced when RJ was added to IVM medium at any supplementation compared with control. Percentage of cleaved embryos and blastocysts was higher in the RJ-treated groups at a concentration of 5 than in the 2.5 mg/ml and control group. Total number of cells per blastocyst was not different in the control and RJ-treated group at 5 mg/ml. However, number of apoptotic cells per blastocyst was higher in the control group than in the RJ-treated group at 5 mg/ml. Expression profile of Bax, and p53 was down-regulated while Bcl-2 was up-regulated in oocytes treated with RJ at 5 and 10 mg/ml compared with the control group. Addition of RJ at concentrations of 5 mg/ml improved embryo production through increasing maturation rate. RJ seems to improve the IVM microenvironment by reducing expression of genes inducing apoptosis, enhancing GSH content, and reducing incidence of apoptosis in blastocysts. |
| Databáze: | OpenAIRE |
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