Transcriptional repression by the Drosophila even-skipped protein: definition of a minimal repression domain
Autor: | Kyuhyung Han, James L. Manley |
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Rok vydání: | 1993 |
Předmět: |
Chloramphenicol O-Acetyltransferase
Saccharomyces cerevisiae Proteins animal structures Proline Transcription Genetic Recombinant Fusion Proteins Blotting Western Molecular Sequence Data Restriction Mapping Protein domain Repressor Biology Fungal Proteins Bacterial Proteins Genetics Animals Drosophila Proteins Amino Acid Sequence Promoter Regions Genetic Transcription factor Psychological repression Cells Cultured Cell Nucleus Homeodomain Proteins Base Sequence Activator (genetics) fungi Genes Homeobox Promoter TATA Box Fusion protein Cell biology DNA-Binding Proteins Gene Expression Regulation Oligodeoxyribonucleotides Drosophila Drosophila Protein Plasmids Transcription Factors Developmental Biology |
Zdroj: | Genes & Development. 7:491-503 |
ISSN: | 1549-5477 0890-9369 |
Popis: | We have used a transient expression assay employing Drosophila tissue culture cells to study the transcriptional repression activity of the homeo domain protein Even-skipped (Eve). Eve was found to repress all promoters that contained Eve-binding sites, including both TATA-containing and TATA-lacking minimal promoters, as well as promoters activated by several different classes of activator proteins. These findings suggest that the general transcription machinery can be a target of Eve. By analyzing properties of a variety of Eve mutants and chimeric fusion proteins, we have identified several features important for efficient repression. In addition to the DNA-binding domain, a potent repressor requires a repression domain, which can be as small as 27 residues. The minimal 57-residue Eve repression domain, as well as several others studied here, were all found to be proline rich and to contain a high percentage of hydrophobic residues. An intriguing feature of the strong repressors was that their DNA-binding activities, measured by gel retention assays with nuclear extracts, were significantly less than those of derivatives inactive in repression. |
Databáze: | OpenAIRE |
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