| Popis: |
Publisher Summary The microscope has been used on many occasions during the earlier studies, but in dealing with bacteria certain points require special attention. Slides must be well cleaned, free from grease, and stored in a stoppered jar containing 50% methylated spirits. If the slides are greasy, it is impossible to make a thin uniform film. The dried smears or films are fixed by passing slowly through a bunsen flame. Sections are prepared and treated in a similar manner to those required for histological examination. Several modifications of the original Romanowsky stains, particularly Leishman's and Giemsa's, are sometimes used for staining bacteria. These stains contain a mixture of polychrome methylene-blue and water-soluble eosin; these form a precipitate, which is washed, dried and dissolved in methyl-alcohol. Giemsa's stain is used for staining spirochaetes. |
