Assessment of the reproductive parameters, laparoscopic oocyte recovery and the first embryos produced in vitro from endangered Canindé goats (Capra hircus)
| Autor: | Joanna Maria Gonçalves Souza-Fabjan, Luciana Magalhães Melo, Carlos H.S. Melo, Vicente José de Figueirêdo Freitas, Eunice Oba, Dárcio Ítalo Alves Teixeira, Deisy J.D. Sanchez, Pascal Mermillod, Alexsandra Fernandes Pereira |
|---|---|
| Přispěvatelé: | Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Laboratory of Physiology and Control of Reproduction, Universidade Federal do Ceará = Federal University of Ceará (UFC), Department of Animal Reproduction and Veterinary Radiology, Universidade de São Paulo (USP), FUNCAP, CNPq e CAPES, Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Universidade Federal do Ceará (UFC) |
| Rok vydání: | 2013 |
| Předmět: |
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
medicine.medical_specialty Conservation of Natural Resources oocyte recovery media_common.quotation_subject Oocyte Retrieval Biology Andrology 03 medical and health sciences Follicle 0302 clinical medicine Endocrinology Pregnancy Internal medicine Capra hircus medicine Medroxyprogesterone acetate Animals Ovulation Progesterone ivp media_common Estrous cycle 030219 obstetrics & reproductive medicine Goats Reproduction goat Endangered Species conservation 0402 animal and dairy science estrus Embryo 04 agricultural and veterinary sciences 040201 dairy & animal science In vitro Breed In Vitro Oocyte Maturation Techniques ovulation Animal Science and Zoology Female Laparoscopy Developmental Biology medicine.drug |
| Zdroj: | Reproductive biology Reproductive biology, 2013, 13 (4), pp.325-332. ⟨10.1016/j.repbio.2013.09.005⟩ |
| ISSN: | 2300-732X 1642-431X |
| DOI: | 10.1016/j.repbio.2013.09.005⟩ |
| Popis: | The Canindé breed of goats (Capra hircus) is currently endangered. The aims of this study were to characterize the estrus behavior, ovulatory responses and progesterone profiles, and to evaluate the in vitro embryo production (IVP) in this breed. In Experiment 1, ten nulliparous and seven pluriparous females received medroxyprogesterone acetate (MAP)-containing sponges (60mg) plus 75μg d-cloprostenol for estrus synchronization and their reproductive parameters were evaluated. In Experiment 2, oocytes obtained by laparascopy from hormonally stimulated females (n=15) were used for IVP. There was no difference (p>0.05) between nulliparous and pluriparous goats in terms of estrus response (40.0% vs. 85.7%), time from progestagen sponge removal to the onset of estrus (62.0±15.5 vs. 50.7±19.2h; mean±SEM), duration of estrus (25.0±16.1 vs. 30.0±15.1h), percentage of ovulating animals (60.0% vs. 85.7%), number of ovulations (1.2±0.4 vs. 1.3±0.8), and diameter of the preovulatory follicle (5.8±0.5 vs. 6.1±0.3mm). Progesterone concentrations were also similar (p>0.05) in both groups. During laparoscopic recovery, there were average 12.2 aspirated follicles and 9.1 oocytes per goat, resulting in a high recovery rate (74.3%, 182/245). A total of 78 embryos were produced (51.0%). The mean number of cells in the blastocysts at day 7 of in vitro culture was 170.3±12.5. In conclusion, nulliparous and pluriparous Canindé goats exhibited similar reproductive profiles. It was possible to produce embryos in vitro, allowing the instigation of an embryo bank for preservation of this breed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect