Ectodermal Wnt-6 promotes Myf5-dependent avian limb myogenesis
| Autor: | Ruijin Huang, Poongodi Geetha-Loganathan, Suresh Nimmagadda, Felicitas Pröls, Martin Scaal, Ketan Patel, Bodo Christ |
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| Rok vydání: | 2005 |
| Předmět: |
Apical ectodermal ridge
animal structures Paraxis Biology MyoD Limb Desmin Avian Proteins Limb bud Myf5 Proto-Oncogene Proteins Ectoderm Basic Helix-Loop-Helix Transcription Factors Animals Paired Box Transcription Factors Limb development Muscle development PAX3 Transcription Factor Molecular Biology Myogenin Muscle Cells Pax3 Myogenesis MyHC Extremities Cell Biology Anatomy musculoskeletal system Cell biology Wnt Proteins Zone of polarizing activity Wnt-6 embryonic structures Chick embryo MYF5 Myogenic Regulatory Factor 5 Signal Transduction Developmental Biology |
| Zdroj: | Developmental Biology. 288:221-233 |
| ISSN: | 0012-1606 |
| DOI: | 10.1016/j.ydbio.2005.09.035 |
| Popis: | Limb muscles of vertebrates are derived from precursor cells that migrate from the lateral edge of the dermomyotome into the limb bud. Although several signaling molecules have been reported to be involved in the process of limb myogenesis, none of their activities has led to a consolidate idea about the limb myogenic pathway. Particularly, the role of ectodermal signals in limb myogenesis is still obscure. Here, we investigated the role of the ectoderm and ectodermal Wnt-6 during limb muscle development. We found that ectopic expression of Wnt-6 in the limb bud specifically extends the expression domains of Pax3, Paraxis, Myf5, Myogenin, Desmin and Myosin heavy chain (MyHC) but inhibits MyoD expression. Ectoderm removal results in a loss of expression of all of these myogenic markers. We show that Wnt-6 can compensate the absence of the ectoderm by rescuing the expression of Pax3, Paraxis, Myf5, Myogenin, Desmin and MyHC but not MyoD. These results show that, in chick, at least two signals from the limb ectoderm are necessary for muscle development. One of the signals is Wnt-6, which plays a unique role in promoting limb myogenesis via Pax3/Paraxis–Myf5, whereas the other putative signaling pathway involving MyoD expression is negatively regulated by Wnt-6 signaling. |
| Databáze: | OpenAIRE |
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