Enantioselective reduction of 3,4-methylene-dioxyphenylacetone using Candida famata and Zygosaccharomyces rouxii
| Autor: | J. Vicenzi, P. Marler, W. Muth, B. Anderson, Milton J. Zmijewski, B. E. Landen |
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| Rok vydání: | 1997 |
| Předmět: |
Ketone
Stereochemistry Alcohol Applied Microbiology and Biotechnology Chemical synthesis Acetone chemistry.chemical_compound Biotransformation Yeasts Food science Candida chemistry.chemical_classification biology Fungi Substrate (chemistry) Stereoisomerism General Medicine Fungi imperfecti biology.organism_classification Yeast Kinetics chemistry Saccharomycetales Oxidation-Reduction Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 47:162-166 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s002530050906 |
| Popis: | In an effort to prepare 3,4-methylene-dioxyphenyl-(S)-isopropanol from 3,4-methylene-dioxyphenylacetone, an initial screen of microbes indicated that Candida famata could catalyze this reaction efficiently at low substrate concentration. A dilute, large-scale process was developed to provide experimental material for the chemical synthesis to be explored. However, the productivity number of this process [0.134 g product (g wet weight cells)-1 day-1 was too low to be practical. C. famata was also extremely sensitive to concentrations of both the ketone and the alcohol greater than 2 g/l. A more extensive screen of yeast and fungi revealed that Zygosaccharomyces rouxii was more tolerant to higher substrate concentrations and had a higher productivity number [0.8 g (g wet weight cells)-1 day-1]. These characteristics suggested that Z. rouxii could be used in a large-scale process at high substrate concentrations. |
| Databáze: | OpenAIRE |
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