Synchrotron radiation microtomography of brain hemisphere and spinal cord of a mouse model of multiple sclerosis revealed a correlation between capillary dilation and clinical score
| Autor: | Shunya Takizawa, Masato Hoshino, Akemi Kamijo, Masato Ohtsuka, Noboru Kawabe, Rino Saiga, Katsuko Naitou, Akihisa Takeuchi, Kentaro Uesugi, Ryuta Mizutani |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Cerebellum Encephalomyelitis Autoimmune Experimental Multiple Sclerosis FOS: Physical sciences Vasodilation Neurological disorder Mice 03 medical and health sciences Imaging Three-Dimensional 0302 clinical medicine Paralysis medicine Animals Physics - Biological Physics business.industry General Neuroscience Multiple sclerosis Experimental autoimmune encephalomyelitis Brain X-Ray Microtomography Spinal cord medicine.disease Physics - Medical Physics Fingolimod Capillaries Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure Spinal Cord Biological Physics (physics.bio-ph) Medical Physics (physics.med-ph) medicine.symptom business Synchrotrons 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Journal of Comparative Neurology. 527:2091-2100 |
| ISSN: | 1096-9861 0021-9967 |
| DOI: | 10.1002/cne.24544 |
| Popis: | Multiple sclerosis is a neurological disorder in which the myelin sheaths of axons are damaged by the immune response. We report here a three-dimensional structural analysis of brain and spinal cord tissues of a mouse model of multiple sclerosis, known as experimental autoimmune encephalomyelitis (EAE). EAE-induced mice were raised with or without administration of fingolimod, which is used in the treatment of multiple sclerosis. Brains and spinal cords dissected from the EAE mice were lyophilized so as to reconstitute the intrinsic contrast of tissue elements, such as axons, in X-ray images. Three-dimensional structures of the brain hemispheres and spinal cords of the EAE mice were visualized with synchrotron radiation microtomography. Microtomographic cross sections reconstructed from the X-ray images revealed dilation of capillary vessels and vacuolation in the spinal cord of the EAE mice. Vacuolation was also observed in the cerebellum, suggesting that the neuroinflammatory response progressed in the brain. The vessel networks and vacuolation lesions in the spinal cords were modelled by automatically tracing the three-dimensional image in order to analyze the tissue structures quantitatively. The results of the analysis indicated that the distribution of vacuolations was not uniform but three-dimensionally localized. The mean vessel diameter showed a linear correlation with the clinical score, indicating that vasodilation is relevant to paralysis severity in the disease model. We suggest that vasodilation and vacuolation are related with neurological symptoms of multiple sclerosis. 22 pages, 5 figures, 1 table |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text