Optimization of Light Intensity, Temperature, and Nutrients to Enhance the Bioactive Content of Hyperforin and Rutin in St. John’s Wort

Autor: Tzu-Shing Deng, Yi-Chin Chou, Chwen-Jen Shieh, Chia-Hung Kuo, Kuo-Chun Liao
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
Light
Pharmaceutical Science
St. John’s wort
melatonin
Secondary metabolite
Phloroglucinol
01 natural sciences
Article
Analytical Chemistry
lcsh:QD241-441
03 medical and health sciences
Rutin
chemistry.chemical_compound
Nutrient
lcsh:Organic chemistry
Drug Discovery
medicine
leaf extracts
hyperforin
Humans
Response surface methodology
Food science
Physical and Theoretical Chemistry
Chromatography
High Pressure Liquid
030304 developmental biology
Active ingredient
0303 health sciences
Plant Extracts
Terpenes
Organic Chemistry
rutin
Temperature
RSM
Hypericum perforatum
Nutrients
Plant Leaves
Light intensity
Hyperforin
chemistry
Chemistry (miscellaneous)
Molecular Medicine
Spectrophotometry
Ultraviolet
optimization
Hypericum
010606 plant biology & botany
medicine.drug
Zdroj: Molecules
Volume 25
Issue 18
Molecules, Vol 25, Iss 4256, p 4256 (2020)
ISSN: 1420-3049
DOI: 10.3390/molecules25184256
Popis: St. John&rsquo
s wort (Hypericum perforatum L.) is a medicinal plant that alleviates depression and other disorders due to its abundance of active ingredients. Hyperforin, rutin, and melatonin are the main active, and important, ingredients in St. John&rsquo
s wort that alleviate depression. In order to investigate the optimal conditions for accumulating these active ingredients, design of experiments and response surface methodology (RSM) was employed in this study. Two-month-old St John&rsquo
s wort plants were cultivated in growth chambers at varying temperatures, light intensities, and nutrient solution concentrations before analysis by HPLC, for determining differences in hyperforin, rutin, and melatonin content. The results showed that hyperforin and rutin contents were significantly influenced by temperature (18&ndash
23 °
C) and light intensity (49&ndash
147 &mu
mol m&minus
2 s&minus
1 photosynthetic photon flux density (PPFD)), whereas Hoagland&rsquo
s nutrient solution concentration (25&ndash
75%) had little effect. The accumulation of melatonin might not be influenced by cultivation conditions. Light intensity and temperature are easily controlled environmental factors in artificial cultivation, both of which are related to secondary metabolite production in the plant. Based on RSM, the optimal conditions for the accumulation of hyperforin and rutin were obtained. The maximum content of hyperforin was 5.6 mg/g, obtained at a temperature of 19 °
C, a nutrient solution concentration of 45%, and a light intensity of 49 &mu
1 PPFD. The maximum content of rutin was 3.8 mg/g obtained at a temperature of 18 °
C, a nutrient solution concentration of 50%, and a light intensity of 147 &mu
1 PPFD. This evaluation of suitable conditions for the accumulation of bioactive compounds in St. John&rsquo
s wort can be applied to plant factories on a large scale.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje