Widespread roles for piRNAs and WAGO-class siRNAs in shaping the germline transcriptome of Caenorhabditis elegans
| Autor: | Taiowa A. Montgomery, Joshua M. Svendsen, Erin Osborne Nishimura, Dustin L. Updike, Tarah Vijayasarathy, Taylor N Marks, Kailee J. Reed, Brooke E Montgomery, Dylan M. Parker, Kristen C. Brown |
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| Rok vydání: | 2019 |
| Předmět: |
Transposable element
endocrine system Piwi-interacting RNA Histones 03 medical and health sciences 0302 clinical medicine RNA interference Gene expression Genetics Animals Gene silencing Gene Silencing RNA Messenger RNA Small Interfering Caenorhabditis elegans Caenorhabditis elegans Proteins RNA Double-Stranded 030304 developmental biology Regulation of gene expression 0303 health sciences biology urogenital system Gene regulation Chromatin and Epigenetics Gene Expression Regulation Developmental High-Throughput Nucleotide Sequencing Argonaute biology.organism_classification Cell biology Germ Cells Argonaute Proteins RNA Interference Transcriptome 030217 neurology & neurosurgery |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | Piwi-interacting RNAs (piRNAs) and small interfering RNAs (siRNAs) are distinct classes of small RNAs required for proper germline development. To identify the roles of piRNAs and siRNAs in regulating gene expression in Caenorhabditis elegans, we subjected small RNAs and mRNAs from the gonads of piRNA and siRNA defective mutants to high-throughput sequencing. We show that piRNAs and an abundant class of siRNAs known as WAGO-class 22G-RNAs are required for proper expression of spermatogenic and oogenic genes. WAGO-class 22G-RNAs are also broadly required for transposon silencing, whereas piRNAs are largely dispensable. piRNAs, however, have a critical role in controlling histone gene expression. In the absence of piRNAs, histone mRNAs are misrouted into the nuclear RNAi pathway involving the Argonaute HRDE-1, concurrent with a reduction in the expression of many histone mRNAs. We also show that high-level gene expression in the germline is correlated with high level 22G-RNA production. However, most highly expressed genes produce 22G-RNAs through a distinct pathway that presumably involves the Argonaute CSR-1. In contrast, genes targeted by the WAGO branch of the 22G-RNA pathway are typically poorly expressed and respond unpredictably to loss of 22G-RNAs. Our results point to broad roles for piRNAs and siRNAs in controlling gene expression in the C. elegans germline. |
| Databáze: | OpenAIRE |
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