Doublex Sequencing in Molecular Diagnosis of Hereditary Diseases
Autor: | H. Voss, W. Ansorge, Jens Plaschke, Matthias Hahn, H.K. Schackert |
---|---|
Rok vydání: | 1998 |
Předmět: |
Genetics
Massive parallel sequencing DNA Mutational Analysis Genes BRCA1 Reproducibility of Results Breast Neoplasms DNA DNA Neoplasm Sequence Analysis DNA Biology Colorectal Neoplasms Hereditary Nonpolyposis Polymerase Chain Reaction Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology DNA sequencing law.invention Single cell sequencing law Hereditary Diseases Humans Primer (molecular biology) Polymerase chain reaction Exome sequencing ABI Solid Sequencing Biotechnology |
Zdroj: | BioTechniques. 24:838-841 |
ISSN: | 1940-9818 0736-6205 |
DOI: | 10.2144/98245dt06 |
Popis: | We describe doublex sequencing of human genomic PCR products using two differently labeled primers in a single reaction and analysis on two automated DNA sequencing devices. Feasibility of the methodology is demonstrated by isothermal and cycle sequencing for two different PCR products and by cycle sequencing on both strands of a single product. It was applied to analyze mutations in patient DNAs in routine sample screening. Because it has the advantage of increased throughput and cost reduction while retaining its accuracy and reading length, we found that doublex sequencing is an attractive option for molecular diagnosis of hereditary diseases. This approach would be even more beneficial if it used DNA sequencing devices with several lasers in a single instrument. |
Databáze: | OpenAIRE |
Externí odkaz: |
načítá se...