High throughput genomic sequencing of bioaerosols in broiler chicken production facilities
| Autor: | Morgan B. Farnell, Joey Lynn Bray, Michael S. Chimenti, Matthew W. Nonnenmann, Kate M. O’Brien, Tom Tabler, Thomas B. Bair |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Dander Indoor bioaerosol Air Microbiology Bioengineering Applied Microbiology and Biotechnology Biochemistry complex mixtures Staphylococcus lentus Microbiology 03 medical and health sciences Abundance (ecology) Animals Humans Food science Animal Husbandry Relative species abundance Soil Microbiology Research Articles Aerosols Lactobacillus crispatus biology Bacteria Lactobacillus salivarius Fungi Computational Biology High-Throughput Nucleotide Sequencing Dust Sequence Analysis DNA biology.organism_classification Archaea Biota respiratory tract diseases 030104 developmental biology Viruses Soil microbiology Chickens Biotechnology Research Article |
| Zdroj: | Microbial Biotechnology |
| ISSN: | 1751-7915 |
| Popis: | Summary Chronic inhalation exposure to agricultural dust promotes the development of chronic respiratory diseases among poultry workers. Poultry dust is composed of dander, chicken feed, litter bedding and microbes. However, the microbial composition and abundance has not been fully elucidated. Genomic DNA was extracted from settled dust and personal inhalable dust collected while performing litter sampling or mortality collection tasks. DNA libraries were sequenced using a paired‐end sequencing‐by‐synthesis approach on an Illumina HiSeq 2500. Sequencing data showed that poultry dust is predominantly composed of bacteria (64–67%) with a small quantity of avian, human and feed DNA ( |
| Databáze: | OpenAIRE |
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