Interval in the replacement of in vitro culture medium affects the integrity and development of equine preantral follicles
| Autor: | Andressa Guidugli Lindquist, Marcelo Marcondes Seneda, Camila Bizarro-Silva, I. Búfalo, S. M. Gonzalez, F. D. Sarapião |
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| Rok vydání: | 2018 |
| Předmět: |
Specific time
equine preantral follicles Ovary Equine Species Biology culture in vitro period Andrology 03 medical and health sciences 0302 clinical medicine Follicular phase medicine horses Developmental stage lcsh:Veterinary medicine 030219 obstetrics & reproductive medicine General Veterinary follicular morphology Significant difference 0402 animal and dairy science Histology In vitro culture 04 agricultural and veterinary sciences 040201 dairy & animal science In vitro medicine.anatomical_structure lcsh:SF600-1100 ovary |
| Zdroj: | Pesquisa Veterinária Brasileira v.38 n.12 2018 Pesquisa Veterinária Brasileira Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA Pesquisa Veterinária Brasileira, Vol 38, Iss 12, Pp 2284-2288 |
| ISSN: | 1678-5150 0100-736X |
| DOI: | 10.1590/1678-5150-pvb-5723 |
| Popis: | The efficiency of a culture system is related to the elaboration and replacement of a medium with conditions suitable for follicular development. Recent investigations suggested that in vitro culture medium should be replaced after specific time periods in various species. However, the suitable interval for the exchange of in vitro culture medium has not yet been established in equine species. The objective of this investigation was to evaluate the effect of medium exchange intervals of 24 hours (T24) or 48 hours (T48) for in vitro culture of preantral follicles at 2 or 6 days. At the end of the culture period, the fragments were processed using classical histology. Equine preantral follicles were classified according to morphological integrity and developmental stage. Data analysis was performed using Fisher’s test with a significance level of p0.05). Compared to the medium replacement at six days of culture, there was a statistically significant difference for T24 (68.9%, p |
| Databáze: | OpenAIRE |
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