Diversity of Cronobacter genus isolated between 1970 and 2019 on the American continent and genotyped using multi-locus sequence typing
| Autor: | Marcelo Luiz Lima Brandão, Paula Vasconcelos Costa, Stephen J. Forsythe, Luiza Vasconcellos |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Veterinary medicine
Databases Factual Microbiology Foodborne Diseases 03 medical and health sciences Cronobacter turicensis Cronobacter sakazakii Cronobacter dublinensis Genetics Humans Typing Cronobacter Molecular Biology 030304 developmental biology 0303 health sciences biology 030306 microbiology Enterobacteriaceae Infections Infant Newborn Cronobacter malonaticus Genetic Variation Infant Peptide Elongation Factor G biology.organism_classification Infant Formula United States Cronobacter muytjensii Multilocus sequence typing Multilocus Sequence Typing |
| Zdroj: | FEMS Microbiology Letters. 368 |
| ISSN: | 1574-6968 |
| DOI: | 10.1093/femsle/fnab027 |
| Popis: | This study aimed to evaluate the Cronobacter spp. strains isolated on the American continent and characterized using multi-locus sequence typing (MLST) available in the PubMLST database and current literature. From 465 Cronobacter spp. strains, the majority (n = 267, 57.4%) was from North America, mainly from USA (n = 234) and 198 (42.6%) were from South America, mainly from Brazil (n = 196). A total of 232 (49.9%) were isolated from foods, 102 (21.9%) from environmental, 87 (18.7%) from clinical, 27 (5.8%) from PIF, one from water (0.2%) and 16 (3.5%) from unknown sources. A total of five species were represented: Cronobacter sakazakii (374, 80.4%), Cronobacter malonaticus (41, 8.8%), Cronobacter dublinensis (29, 6.2%), Cronobacter turicensis (16, 3.5%) and Cronobacter muytjensii (5, 1.1%). The strains with complete MLST profile (n = 345) were assigned to 98 STs, a ratio of 3.5 strain by ST found and the calculated Simpson`s index was 0.93. The strains showed a high diversity and after eBURST analysis, 30 STs (n = 189) formed 12 single and/or double-locus variant clonal complexes (CC). A total of 38 STs (38.7%) were associated with clinical cases of infection, including well established C. sakazakii CC 1, 4, 8 and 83; C. malonaticus ST60, 307, 394 and 440; and C. sakazakii ST 12 and 494. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|